In Vitro: Pretreatment with Unoprostone (0.01, 0.1, 1 μM; 1 h before H2O2 treatment) protects against H2O2-induced cell death in a concentration-dependent manner and the effect is significant at 0.1 μM and 1 μM concentrations. Pretreatment with Unoprostone at concentrations of 0.1 to 3 μM protects against light-induced cell death in a concentration-dependent manner; the effect is significant at the 1 and 3 μM concentrations. Unoprostone reduces the morphological change, and it significantly inhibits the low mitochondrial membrane potential and cell death induced by light irradiation. Unoprostone has prostaglandin F2α receptors (FP) binding affinity with a Ki of 3.86 μM.
In Vivo: Unoprostone (0.06%, 0.12%; topical instillation; 3 µl) reduces mouse intraocular pressure (IOP) in a dose-dependent manner.