RIM 2

Loose-Patch Voltage-Clamp Technique

tubing. With this method, discrete areas of membrane can be sampled by pressing the rim of the patch pipet against the cell membrane to electrically isolate it from the external saline. Early experiments focally stimulated discrete areas

Confocal Microscopy of Adipocytes

in biochemical studies, the unique structure of the adipose cell, with its large central triglyceride storage droplet (80 μm) and thin (1–2 μm) rim of cytoplasm, has caused special problems for morphological approaches. Several methods have been used for studies

Pipette Internal Perfusion: Methods and Applications

and current-clamp conditions. Cells can be patched in a variety of configurations. Whole-cell currents and voltage can be measured by forming a high resistance seal between the cell membrane and the rim of the glass or quartz patch