日本JCRB细胞

日本JCRB细胞库，专注于收集各种人类和动物培养细胞，包括癌症和转基因细胞。 细胞资源被分配给日本乃至全世界的研究人员。 这些细胞通过测试微生物污染，病毒污染和交叉培养物污染而获得全面认证。 一些细胞的特征是核型分析和/或细胞表面标记。 我们与世界上其他主要的细胞库合作，正在开发细胞培养和质量控制方法，以支持药物科学医学的基础研究。

We, JCRB Cell Bank, are collecting various human and animal culture cells including cancer and genetically modified cells. Cell resources are distributed to researchers across Japan and around the world. These cells are comprehensively qualified by testing microbial contamination, virus contamination and cross culture contamination. Some cells are characterized by karyotyping and/or cell surface markers. In collaboration with other major cell banks in the world, we are developing methods for cell culturing and quality control in order to support fundamental research on medical of pharmaceutical sciences.



我们优势进口日本JCRB的细胞等产品，价格优惠，货期6-8周，低温产品提供全程干冰运输服务，质量和售后有保障！


以JCRB0820，EBC-1细胞为例，产品信息如下：

Cell type：general cells

JCRB No.	JCRB0820	Cell Name	EBC-1
Profile	squamous cell carcinoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	69
Identity	available	Tissue for Primary Cancer	lung (cancer)
Case history		Metastasis	No
Tissue Metastasized		Genetics	squamous cell carcinoma
Life Span		Crisis PDL
Morphology		Character	squamous cell carcinoma
Classify		Established by	Hiraki,S.
Registered by	Akiyama,M.	Regulation for Distribution
Comment		Year	1987
Medium	Eagle's minimal essential medium with 10% fetal calf serum	Methods for Passages	0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage	split ratio=1/6	Race
CO2 Conc.	5 %	Tissue Sampling	lung
Tissue Type

 

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

 

Link
Cellosaurus(ExPASy)	CVCL_2891

 

Reference
Pubmed id:2736210	Production of transforming growth factor-alpha in human tumour cell lines. Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K Br J Cancer. 1989 May;59(5):761-5
Pubmed id:none	Establishment of human continuous cell lines from squamous cell, adeno- and small cell carcinoma of the lung and the results of hetero-transplantation Hiraki S, Miyai M, Seto T, Tamura T, Watanabe Y, Ozawa S, Ikeda H, Nakata Y, Ohnoshi T, Kimura I Haigan. 1982 22(1):53-8

细胞图片：






 

Cell No.	JCRB0820	Cell Name	EBC-1
LOT No.	08012011	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	6.2 - 12 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	95.3	Antibiotics Used	free
Passage Number	P36	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS -EMEM	CO2 Conc.	5%
RFLP		Viability immediately after thawing (%)
Additional information	Expected viability (defrost ampoule) 97 %.

从美国进口细胞订购流程：

Sekisui XenoTech will handle the importation of your cell order from JCRB and ship via FedEx Overnight inside the domestic US and Canada.
Pricing for various cell types are as follows.

Cell type	End user's affiliation
	Non-Profit Organization	Profit Organization
general cells	$740	$790
human iPS cells	Coming Soon	Coming Soon
luciferase-expressing cancer cells	$800	$850
mousehomozygous mutant ES cells	$800	$850
human mutant cell repository from Kyoto University	not available	not available
virus production cells	not available	not available
primary mesenchymal stem cell	not available	not available
immortalized mesenchymal stem cell	$740	$790
genetically-modified cells	$800	$850
CITES regulated cells	$900	$950

Cells will be shipped from Sekisui XenoTech’s Global Headquarters in Kansas City, KS. Please check with Sekisui XenoTech prior to ordering if you have specific questions regarding delivery timelines.

All cell orders will be shipped overnight on dry ice via FedEx.

All cell pricing is inclusive of shipping & handling fees to your laboratory. There will be no additional fees added to your order at the time of processing.

Sekisui XenoTech has teamed up with the National Institute of Biomedical Innovation to bring you access to roughly 1,100 cell lines worldwide. Sekisui XenoTech is the exclusive North American provider of JCRB’s comprehensive catalog of cell lines. 
Sekisui XenoTech, LLC
1101 West Cambridge Circle Dr.
Kansas City, KS 66103
USA

Phone: (913) 438-7450
Fax: (913) 227-7100
General Information: info at xenotechllc.com
http://，www.xenotech.com/ 

If you are interested in purchasing a cell line offered by JCRB, please proceed to order online through the JCRB website and Sekisui XenoTech will take care of the rest. 

All pricing on the JCRB website is inclusive of shipping & handling charges to your North American laboratory location. 

Please note that while you are placing your order request online through JCRB, Sekisui XenoTech will be handling all aspects of your order, so if you have any questions, please contact us at info at xenotechllc.com.

If for any reason, the purchaser wishes to cancel a pending order, they must adhere to this cancellation policy. All order cancellation requests must be made PRIOR to JCRB’s shipment of the order to Sekisui XenoTech. Once an order has been shipped from JCRB to Sekisui XenoTech, that order is no longer eligible for cancellation or refund. Shipments from JCRB to Sekisui XenoTech occur bi-weekly on Friday. If you have specific questions pertaining to the shipping calendar or regarding this policy, please contact a Sekisui XenoTech representative.