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squamous cell carcinoma
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69
干冰、液氮
lung
是
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人
lung (cancer)
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100
EBC-1
JCRB0820
北京孚博
1支
JCRB No. | JCRB0820 | Cell Name | EBC-1 |
Profile | squamous cell carcinoma | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | M | Age | 69 |
Identity | available | Tissue for Primary Cancer | lung (cancer) |
Case history | Metastasis | No | |
Tissue Metastasized | Genetics | squamous cell carcinoma | |
Life Span | Crisis PDL | ||
Morphology | Character | squamous cell carcinoma | |
Classify | Established by | Hiraki,S. | |
Registered by | Akiyama,M. | Regulation for Distribution | |
Comment | Year | 1987 | |
Medium | Eagle's minimal essential medium with 10% fetal calf serum | Methods for Passages | 0.25% trypsin and dilute in fresh medium. Change medium twice a week |
Cell Number on Passage | split ratio=1/6 | Race | |
CO2 Conc. | 5 % | Tissue Sampling | lung |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - | -/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Link | |
Cellosaurus(ExPASy) | CVCL_2891 |
Reference | |
Pubmed id:2736210 | Production of transforming growth factor-alpha in human tumour cell lines. Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K Br J Cancer. 1989 May;59(5):761-5 |
Pubmed id:none | Establishment of human continuous cell lines from squamous cell, adeno- and small cell carcinoma of the lung and the results of hetero-transplantation Hiraki S, Miyai M, Seto T, Tamura T, Watanabe Y, Ozawa S, Ikeda H, Nakata Y, Ohnoshi T, Kimura I Haigan. 1982 22(1):53-8 |
Cell No. | JCRB0820 | Cell Name | EBC-1 |
LOT No. | 08012011 | Lot Specification | distribution |
Medium | Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
Cell Density at Seeding | 6.2 - 12 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | approx. 32 hrs. | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
Viability at cell freezing (%) | 95.3 | Antibiotics Used | free |
Passage Number | P36 | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS -EMEM | CO2 Conc. | 5% |
RFLP | Viability immediately after thawing (%) | ||
Additional information | Expected viability (defrost ampoule) 97 %. |
Cell type | End user's affiliation | |
Non-Profit Organization | Profit Organization | |
general cells | $740 | $790 |
human iPS cells | Coming Soon | Coming Soon |
luciferase-expressing cancer cells | $800 | $850 |
mousehomozygous mutant ES cells | $800 | $850 |
human mutant cell repository from Kyoto University | not available | not available |
virus production cells | not available | not available |
primary mesenchymal stem cell | not available | not available |
immortalized mesenchymal stem cell | $740 | $790 |
genetically-modified cells | $800 | $850 |
CITES regulated cells | $900 | $950 |
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