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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
50
- 保质期:
6个月
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
2-8℃,6个月
- 规格:
500mL
Washing procedures are critical steps in the preparation of platelets for experimental research, since integrity and function of platelets may be affected. This study investigated the initial effects of washing platelets on ADP-mediated platelet responsiveness in comparison with freshly prepared PRP in order to evaluate molecular and functional alterations in washed platelets. As a common medium, CGS buffer was used for the preparation of washed platelets. Subsequent storage of PRP and of washed platelets (suspended in HEPES buffer) was performed under equal conditions at 22°C without agitation.
with sodium chloride, D-glucose, trisodium citrate
References: Role of Purinergic Receptor Expression and Function for Reduced Responsiveness to Adenosine Diphosphate in Washed Human Platelets PMID: 26808867
常温运输,2-8℃,6个月
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文献和实验Buffer 配方 1.0 mol/L Tris•HCl(pH 6.8) 溶解后,用浓盐酸调 pH 至 6.8,最后用超纯水定容至 250 mL,高温灭菌后室温下保存。 1.5 mol/L Tris•HCl(pH 8.8) 溶解后,用浓盐酸调 pH 至 8.8,最后用超纯水定容至 250mL,高温灭菌后室温下保存。 10% SDS 取 SDS 10g,加蒸馏水至 100 mL, 50 ℃ 水浴下溶解,室温保存。如在长期保存中出现沉淀,水浴溶化后,仍可使用。 10% 过硫酸胺(APS) 取
一般弱酸或弱碱与其盐(强电解质)的混合物显有缓冲作用,具有这种作用的系统称为缓冲系统。在缓冲系统中虽有的以固态加入,但大多数情况全部物质都进行溶解,此称为缓冲液( buffer solution)。在考虑弱酸 HA及其盐 MA的混合液的 pH时,首先在弱酸 HA有如下的平衡式成立: 这里 []表示各自的浓度, K表示弱酸的解离常数。现假定弱酸 HA的浓度为 Cn ,盐 MA的浓度为 Cm ,那末,未解离的酸的浓度 [HA]=Cn -[H
Buffer Formula (required precision; 2 %) Always to try to prepare buffer solution at the temperature and concentration before planing to use during the experiment. Stock solutions are acceptable as long as pH adjustment
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