基因
种属:2019-nCoV
NCBI 参考序列号:NC_045512.2(26245-26472nt)
基因长度:228 bp
序列特征:Identical with the Gene Bank Ref. ID sequence.
产品特征:Full length Clone DNA of SARS-CoV-2 (2019-nCoV) envelope protein.
质粒
启动子:Enhanced CMV mammalian cell promoter
载体:pCMV3-untagged
限制性酶切位点:KpnI + NotI(6.1kb+0.23kb)
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控:The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性:Ampicillin
细胞筛选抗性:Hygromycin
应用:Stable or Transient mammalian expression
1, Han, C; et al. Characterization of extracellular vesicle and virus-like particles by single vesicle tetraspanin analysis. Sensors and Actuators B: Chemical