- ￥1770
- Sino Biological
- 北京
- VG40588-CF
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
SARS-CoV-2 (2019-nCoV) Nucleoprotein Gene ORF cDNA clone expression plasmid, C-FLAG tag (Codon Optimized)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
基因
种属：2019-nCoV
基因长度：1299 bp
序列特征：A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with QHD43423.2 except for the point mutations: G335A.
产品特征：Full length Clone DNA of SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP.
质粒
启动子：Enhanced CMV mammalian cell promoter
载体：pCMV3-C-FLAG
限制性酶切位点：KpnI + XbaI(6kb+1.3kb)
标签序列：FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物：T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控：The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性：Kanamycin
细胞筛选抗性：Hygromycin
应用：Stable or Transient mammalian expression
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文献和实验The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
45a and Jnk1 expression in the vital organs of CBA/CA mice. In Vivo 25:241‐244. Kash, J.C., Tumpey, T.M., Proll, S.C., Carter, V., Perwitasari, O., Thomas, M.J., Basler, C.F
Site-directed hydroxy radical mapping of nucleosome positions in vitro
octamer at 0-4°C, where it is typically stable for at least a month. Preparation of Labelled DNA We routinely prepare DNA fragments for mono- and di-nucleosomes made by preparative PCR and ion exchange purification (Bruno et al ., 2004
