基因
种属:2019-nCoV
基因长度:1299 bp
序列特征:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with QHD43423.2 except for the point mutations: G335A.
产品特征:Full length Clone DNA of SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP.
质粒
启动子:Enhanced CMV mammalian cell promoter
载体:pCMV3-C-FLAG
限制性酶切位点:KpnI + XbaI(6kb+1.3kb)
标签序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控:The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性:Kanamycin
细胞筛选抗性:Hygromycin
应用:Stable or Transient mammalian expression