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- 信裕生物
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- 2025年07月13日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
Acetic Acid-Sodium Acetate Buffer
- 保质期：
12个月
- 供应商：
上海信裕生物科技有限公司
- 保存条件：
常温运输与保存。
- 规格：
500mL
缓冲溶液作用原理和pH值
当往某些溶液中加入一定量的酸和碱时，有阻碍溶液pH变化的作用，称为缓冲作用，这样的溶液叫做缓冲溶液。弱酸及其盐的混合溶液（如HAc与NaAc），弱碱及其盐的混合溶液（如NH3·H2O与NH4Cl）等都是缓冲溶液。
由弱酸HA及其盐NaA所组成的缓冲溶液对酸的缓冲作用，是由于溶液中存在足够量的碱A-的缘故。当向这种溶液中加入一定量的强酸时，H 离子基本上被A-离子消耗：
所以溶液的pH值几乎不变；当加入一定量强碱时，溶液中存在的弱酸HA消耗OH-离子而阻碍pH的变化。
Many important reactions in chemistry and biochemistry are pH-dependent, meaning that the pH of the solution can play an important role in determining whether and how rapidly a reaction takes place. Consequently, buffers---solutions that help keep the pH stable---are important for running many experiments. Sodium acetate is a weakly basic salt and the conjugate base of acetic acid, or vinegar. A mixture of sodium acetate and acetic acid makes a good buffer for weakly acidic solutions. A few different ways exist to prepare an acetate buffer, but one method in particular is straightforward and relatively safe.
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文献和实验Sodium Acetate Precipitation of Small Nucleic Acids
实验步骤 1. Add 2 μl carrier to the nucleic acid solution and mix well. 2. Add 1:10 volume of 3 M sodium acetate and mix thoroughly; for 230 μl of Lower Running Buffer, this will be 23 μl of 3 M
Silver Acetate Autometallography (AMG)
of glass double-distilled water. (Silver acetate crystals can be dissolved by continuous stirring within about 15 min.) Citrate buffer: Dissolve 23.5 g of trisodium citrate dihydrate and 25.5 g citric acid monohydrate in 850 mL or deionized
Phosphoamino acid analysis:Mark Kamps's method
of each buffer. pH 1.9 buffer. 88% Formic acid 50 ml Acetic acid 156 ml H2O 1794 ml Don't use the 98% formic acid and don't adjust the pH. pH 3.5 buffer Pyridine 10 ml Acetic acid 100 ml 100 mmolar EDTA 10 ml H2O 1880 ml Don't bother to adjust
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