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文献和实验for this purpose. ::Chaotrophe: urea and thiourea::Reductant: DTT, Tris(2-carboxyethyl) phosphine (TCEP), tributyl phosphine (TBP)::Detergent: CHAPS, triton x-100::Ampholytes::Protease inhibitors Some researchers also add tris buffer.Chaotrophe.Urea
buffers we use are listed below, obviously there are other reductants and detergents that can be used for this purpose.:: Chaotrophe: urea and thiourea:: Reductant: DTT, Tris(2-carboxyethyl) phosphine (TCEP), tributyl phosphine (TBP):: Detergent
丙烯酰胺胶中肽提取和蛋白消化技术Digestion of Proteins and Extraction of Peptides from an Acrylamide Gel
fresh) K3 Fe(CN)6 Na2 S2 O3 ・ 5H2 O Proteins in gel slices as obtained in Protocol 1, contained in a microfuge tube Tris (2-carboxyethyl)-phosphine hydrochloride (TCEP) Trypsin
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