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文献和实验them down and continue. These pellets do not hold together very well so tke care that they do not fall apart in the resin. 12). Leave in fresh propylene oxide for 20' then in propylene oxide/Spurr's resin (1:1 mix) overnight. Mixing during the incubation will help
Immunofluorescence on ultrathin resin sections
. Residual water is drained with filter paper along the outside of the loop. Note: air-dried resin sections can be stored for months prior to labelling. Procedure: Encircle sections
Coupling Peptides To Resin For Affinity Purification
We use Affigel-10, converting its functional group first to amino and then to iodoacetyl. You can also buy amino resin (it's more expensive) and start at step 6. All washes are performed on a glass-fritted filter
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