- ￥3284
- CHI Scientific
- 8-80117
- USA
- 2025年12月15日
- immunostaining, westernblot, immunoprecipitation.
- rabbit
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- 文献和实验
- 技术资料
- 供应商：
齐氏生物
- 库存：
100 μg
- 靶点：
详询
- 级别：
详询
- 目录编号：
8-80114
- 克隆性：
多克隆
- 抗原来源：
synthetic peptide antigen
- 保质期：
6 months from date of receipt
- 抗体英文名：
anti-hER-α66
- 抗体名：
anti-hER-α66抗体
- 标记物：
详询
- 宿主：
rabbit
- 适应物种：
详询
- 免疫原：
详询
- 亚型：
详询
- 形态：
Liquid
- 应用范围：
immunostaining, westernblot, immunoprecipitation.
- 浓度：
1mg/ml
- 保存条件：
2-8° for short term; freeze in Suitable Aliquots
- 规格：
100 μg
产品说明
Descriptions: The antibody was raised against a synthetic peptide antigen corresponding to the unique C-terminal 18 amino acids of hER-α66. The specificity of the antibody was tested in hER-α66 expression cells, MCF-7 Cells and breast carcinoma tissues. This rabbit polyclonal anti-hER-α66 antibody is supplied in affinity-purified form.
Storage: 2-8 ℃ for short term; freeze in Suitable Aliquots.
Antibody Concentration: 1 mg/ml.
Physical Appearance: Liquid.
Buffer: Tris/Glycine (pH7.4).
Stabilizing Agent: 0.1% BSA.
Preservative: in 10mM PBS, pH 7.6, with 0.2% BSA and 15mM sodium azide.
Shelf Life: 6 months from date of receipt.
Reactivity: human, mouse, and hamster (not rat ,others not tested)
Application: immunostaining, westernblot, immunoprecipitation.
Storage: 2-8 ℃ for short term; freeze in Suitable Aliquots.
Antibody Concentration: 1 mg/ml.
Physical Appearance: Liquid.
Buffer: Tris/Glycine (pH7.4).
Stabilizing Agent: 0.1% BSA.
Preservative: in 10mM PBS, pH 7.6, with 0.2% BSA and 15mM sodium azide.
Shelf Life: 6 months from date of receipt.
Reactivity: human, mouse, and hamster (not rat ,others not tested)
Application: immunostaining, westernblot, immunoprecipitation.
规格型号
论文引用（2007-2019部分）
(图片选自文章： https://www.jbc.org/content/282/37/27478/F9.expansion.html）
1.Estrogen signaling and estrogen receptors as prognostic indicators in laryngeal cancer
A Verma, N Schwartz, DJ Cohen, BD Boyan… - Steroids, 2019 - Elsevier
… Abcam, Cambridge, United Kingdom) or ERα (rabbit polyclonal, Chi Scientific, Maynard, MA),
which detects ERα66 and ERα36. All tissue or cell lysates were run on two separate western
blots, and data from one representative blot is shown. 2.8.1. Estrogen and estrogen …
Cited by 2 Related articles All 5 versions
2.24R, 25-dihydroxyvitamin D3 modulates tumorigenicity in breast cancer in an estrogen receptor-dependent manner
A Verma, Z Schwartz, BD Boyan - Steroids, 2019 - Elsevier
… Western blots were used to identify and quantify estrogen receptor protein in all cell lines as
previously described [9]. In brief, cells were grown to confluence in T …ERα66, ERα46, and ERα36
were assessed with an all-ERα primary antibody (Chi Scientific #80113, Maynard, MA) …
3.Effect of 17β-estradiol on estrogen receptor negative breast cancer cells in an osteolytic mouse model
DJ Cohen, V Patel, A Verma, BD Boyan, Z Schwartz - Steroids, 2019 - Elsevier
… Effects of estrogen on osteolysis were assessed as a function of time and treatment with
17β-estradiol. 2. Material and methods … Primary antibodies against ERα were from Chi Scientific
(Maynard, MA, #8-80119, #8-80113); antibodies to GAPDH were from Millipore-Sigma (St …
Cited by 1 Related articles All 6 versions
4.Estradiol receptor profile and estrogen responsiveness in laryngeal cancer and clinical outcomes
N Schwartz, A Verma, C Muktipaty, C Bivens… - Steroids, 2019 - Elsevier
… Louis, MO, #C4555-10G) [16]. Estrogen receptors were inhibited using a neutralizing antibody
to ERα36 (Chi Scientific, Maynard, MA) [37]. Confluent cultures were pre-treated with media
containing different concentrations of the inhibitor as indicated below …
Cited by 6 Related articles All 5 versions
5.Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism
RA Chaudhri, A Hadadi, KS Lobachev… - … et Biophysica Acta (BBA …, 2014 - Elsevier
… Estrogen receptors (ERs) play a major role in classification, diagnosis, and treatment of breast
cancer [13], [14], [15] … ERα36 overexpression plasmids were purchased from Chi Scientific
(Maynard, MA). Polyfect transfection reagent was obtained from Qiagen (Germantown, MD) …
6.Membrane estrogen signaling enhances tumorigenesis and metastatic potential of breast cancer cells via estrogen receptor-α36 (ERα36)
RA Chaudhri, R Olivares-Navarrete, N Cuenca… - Journal of Biological …, 2012 - ASBMB
… needed. The purpose of this study was to evaluate the role of ERα36 in membrane-
associated estrogen signaling in breast cancer … Sciences. Polyclonal ERα66 and ERα36
antibodies were purchased from Chi Scientific (Maynard, MA) …
Cited by 90 Related articles All 8 versions
7.Regulation of Mouse Slo Gene Expression MULTIPLE PROMOTERS, TRANSCRIPTION START SITES, AND GENOMIC ACTION OF ESTROGEN
P Kundu, A Alioua, E Stefani, L Toro - Journal of Biological Chemistry, 2007 - ASBMB
… Estrogen-treated MCF7 cell nuclear extract was from Active Motif … of nuclear extract from HeLa
cells transfected with hERα was resolved in a 4-20% gradient SDS gel and immunoblotted using
a polyclonal antibody raised against hERα (ER-α66, CHI Scientific) as described …
Cited by 46 Related articles All 6 versions
更多抗体：
代表产品
论文引用（2007-2019部分）
(图片选自文章： https://www.jbc.org/content/282/37/27478/F9.expansion.html）
1.Estrogen signaling and estrogen receptors as prognostic indicators in laryngeal cancer
A Verma, N Schwartz, DJ Cohen, BD Boyan… - Steroids, 2019 - Elsevier
… Abcam, Cambridge, United Kingdom) or ERα (rabbit polyclonal, Chi Scientific, Maynard, MA),
which detects ERα66 and ERα36. All tissue or cell lysates were run on two separate western
blots, and data from one representative blot is shown. 2.8.1. Estrogen and estrogen …
Cited by 2 Related articles All 5 versions
2.24R, 25-dihydroxyvitamin D3 modulates tumorigenicity in breast cancer in an estrogen receptor-dependent manner
A Verma, Z Schwartz, BD Boyan - Steroids, 2019 - Elsevier
… Western blots were used to identify and quantify estrogen receptor protein in all cell lines as
previously described [9]. In brief, cells were grown to confluence in T …ERα66, ERα46, and ERα36
were assessed with an all-ERα primary antibody (Chi Scientific #80113, Maynard, MA) …
3.Effect of 17β-estradiol on estrogen receptor negative breast cancer cells in an osteolytic mouse model
DJ Cohen, V Patel, A Verma, BD Boyan, Z Schwartz - Steroids, 2019 - Elsevier
… Effects of estrogen on osteolysis were assessed as a function of time and treatment with
17β-estradiol. 2. Material and methods … Primary antibodies against ERα were from Chi Scientific
(Maynard, MA, #8-80119, #8-80113); antibodies to GAPDH were from Millipore-Sigma (St …
Cited by 1 Related articles All 6 versions
4.Estradiol receptor profile and estrogen responsiveness in laryngeal cancer and clinical outcomes
N Schwartz, A Verma, C Muktipaty, C Bivens… - Steroids, 2019 - Elsevier
… Louis, MO, #C4555-10G) [16]. Estrogen receptors were inhibited using a neutralizing antibody
to ERα36 (Chi Scientific, Maynard, MA) [37]. Confluent cultures were pre-treated with media
containing different concentrations of the inhibitor as indicated below …
Cited by 6 Related articles All 5 versions
5.Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism
RA Chaudhri, A Hadadi, KS Lobachev… - … et Biophysica Acta (BBA …, 2014 - Elsevier
… Estrogen receptors (ERs) play a major role in classification, diagnosis, and treatment of breast
cancer [13], [14], [15] … ERα36 overexpression plasmids were purchased from Chi Scientific
(Maynard, MA). Polyfect transfection reagent was obtained from Qiagen (Germantown, MD) …
6.Membrane estrogen signaling enhances tumorigenesis and metastatic potential of breast cancer cells via estrogen receptor-α36 (ERα36)
RA Chaudhri, R Olivares-Navarrete, N Cuenca… - Journal of Biological …, 2012 - ASBMB
… needed. The purpose of this study was to evaluate the role of ERα36 in membrane-
associated estrogen signaling in breast cancer … Sciences. Polyclonal ERα66 and ERα36
antibodies were purchased from Chi Scientific (Maynard, MA) …
Cited by 90 Related articles All 8 versions
7.Regulation of Mouse Slo Gene Expression MULTIPLE PROMOTERS, TRANSCRIPTION START SITES, AND GENOMIC ACTION OF ESTROGEN
P Kundu, A Alioua, E Stefani, L Toro - Journal of Biological Chemistry, 2007 - ASBMB
… Estrogen-treated MCF7 cell nuclear extract was from Active Motif … of nuclear extract from HeLa
cells transfected with hERα was resolved in a 4-20% gradient SDS gel and immunoblotted using
a polyclonal antibody raised against hERα (ER-α66, CHI Scientific) as described …
Cited by 46 Related articles All 6 versions
更多抗体：
代表产品
| 分类 | 抗体名称 |
| 单克隆抗体 | Anti-KDM5C mouse monoclonal antibody Anti-KDM5C mouse monoclonal antibody Anti-GTF2B mouse monoclonal antibody Anti-SMYD2 mouse monoclonal antibody Anti-CXCL8 mouse monoclonal antibody Anti-SMC1A(N-term) mouse monoclonal antibody Anti-MAP2(N-term) mouse monoclonal antibody Anti-BRAF mouse monoclonal antibody Anti-GRK2 mouse monoclonal antibody Anti-FOXA1 mouse monoclonal antibody Anti-GATA4 mouse monoclonal antibody Anti-BCL2L1 mouse monoclonal antibody |
| 多克隆抗体 | Anti-PCNA rabbit polyclonal antibody Anti-STAT1 rabbit polyclonal antibody Anti-MMP1 rabbit polyclonal antibody Anti-TLR2 rabbit polyclonal antibody Anti-ANXA1 rabbit polyclonal antibody Anti-KRT8 rabbit polyclonal antibody Anti-ATF2 rabbit polyclonal antibody Anti-CAPN1 rabbit polyclonal antibody Anti-CCK rabbit polyclonal antibody Anti-CLEC10A rabbit polyclonal antibody Anti-EFNA3 rabbit polyclonal antibody Anti-TNFRSF10C rabbit polyclonal antibody |
| 标签/ 内参抗体 |
Anti-GAPDH mouse monoclonal antibody Anti-ACTB(HRP-Conjugate) mouse monoclonal antibody Anti-TUBB(HRP-Conjugate) mouse monoclonal antibody Anti-Rubisco mouse monoclonal antibody Anti-Myc-Tag Mouse Monoclonal antibody Anti-GST Tag mouse monoclonal antibody Anti-Trx-Tag mouse monoclonal antibody Anti-DsbA-Tag mouse monoclonal antibody Anti-GFP/eGFP-Tag mouse monoclonal antibody Anti-Flag-Tag mouse monoclonal antibody Anti-6×His Tag mouse monoclonal antibody Anti-MBP Tag mouse monoclonal antibody Anti-EYFP Tag mouse monoclonal antibody |
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文献和实验该产品被引用文献
1.Estrogen signaling and estrogen receptors as prognostic indicators in laryngeal cancer
A Verma, N Schwartz, DJ Cohen, BD Boyan… - Steroids, 2019 - Elsevier
… Abcam, Cambridge, United Kingdom) or ERα (rabbit polyclonal, Chi Scientific, Maynard, MA),
which detects ERα66 and ERα36. All tissue or cell lysates were run on two separate western
blots, and data from one representative blot is shown. 2.8.1. Estrogen and estrogen …
Cited by 2 Related articles All 5 versions
2.24R, 25-dihydroxyvitamin D3 modulates tumorigenicity in breast cancer in an estrogen receptor-dependent manner
A Verma, Z Schwartz, BD Boyan - Steroids, 2019 - Elsevier
… Western blots were used to identify and quantify estrogen receptor protein in all cell lines as
previously described [9]. In brief, cells were grown to confluence in T …ERα66, ERα46, and ERα36
were assessed with an all-ERα primary antibody (Chi Scientific #80113, Maynard, MA) …
3.Effect of 17β-estradiol on estrogen receptor negative breast cancer cells in an osteolytic mouse model
DJ Cohen, V Patel, A Verma, BD Boyan, Z Schwartz - Steroids, 2019 - Elsevier
… Effects of estrogen on osteolysis were assessed as a function of time and treatment with
17β-estradiol. 2. Material and methods … Primary antibodies against ERα were from Chi Scientific
(Maynard, MA, #8-80119, #8-80113); antibodies to GAPDH were from Millipore-Sigma (St …
Cited by 1 Related articles All 6 versions
4.Estradiol receptor profile and estrogen responsiveness in laryngeal cancer and clinical outcomes
N Schwartz, A Verma, C Muktipaty, C Bivens… - Steroids, 2019 - Elsevier
… Louis, MO, #C4555-10G) [16]. Estrogen receptors were inhibited using a neutralizing antibody
to ERα36 (Chi Scientific, Maynard, MA) [37]. Confluent cultures were pre-treated with media
containing different concentrations of the inhibitor as indicated below …
Cited by 6 Related articles All 5 versions
5.Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism
RA Chaudhri, A Hadadi, KS Lobachev… - … et Biophysica Acta (BBA …, 2014 - Elsevier
… Estrogen receptors (ERs) play a major role in classification, diagnosis, and treatment of breast
cancer [13], [14], [15] … ERα36 overexpression plasmids were purchased from Chi Scientific
(Maynard, MA). Polyfect transfection reagent was obtained from Qiagen (Germantown, MD) …
6.Membrane estrogen signaling enhances tumorigenesis and metastatic potential of breast cancer cells via estrogen receptor-α36 (ERα36)
RA Chaudhri, R Olivares-Navarrete, N Cuenca… - Journal of Biological …, 2012 - ASBMB
… needed. The purpose of this study was to evaluate the role of ERα36 in membrane-
associated estrogen signaling in breast cancer … Sciences. Polyclonal ERα66 and ERα36
antibodies were purchased from Chi Scientific (Maynard, MA) …
Cited by 90 Related articles All 8 versions
7.Regulation of Mouse Slo Gene Expression MULTIPLE PROMOTERS, TRANSCRIPTION START SITES, AND GENOMIC ACTION OF ESTROGEN
P Kundu, A Alioua, E Stefani, L Toro - Journal of Biological Chemistry, 2007 - ASBMB
… Estrogen-treated MCF7 cell nuclear extract was from Active Motif … of nuclear extract from HeLa
cells transfected with hERα was resolved in a 4-20% gradient SDS gel and immunoblotted using
a polyclonal antibody raised against hERα (ER-α66, CHI Scientific) as described …
Cited by 46 Related articles All 6 versions
A Verma, N Schwartz, DJ Cohen, BD Boyan… - Steroids, 2019 - Elsevier
… Abcam, Cambridge, United Kingdom) or ERα (rabbit polyclonal, Chi Scientific, Maynard, MA),
which detects ERα66 and ERα36. All tissue or cell lysates were run on two separate western
blots, and data from one representative blot is shown. 2.8.1. Estrogen and estrogen …
Cited by 2 Related articles All 5 versions
2.24R, 25-dihydroxyvitamin D3 modulates tumorigenicity in breast cancer in an estrogen receptor-dependent manner
A Verma, Z Schwartz, BD Boyan - Steroids, 2019 - Elsevier
… Western blots were used to identify and quantify estrogen receptor protein in all cell lines as
previously described [9]. In brief, cells were grown to confluence in T …ERα66, ERα46, and ERα36
were assessed with an all-ERα primary antibody (Chi Scientific #80113, Maynard, MA) …
3.Effect of 17β-estradiol on estrogen receptor negative breast cancer cells in an osteolytic mouse model
DJ Cohen, V Patel, A Verma, BD Boyan, Z Schwartz - Steroids, 2019 - Elsevier
… Effects of estrogen on osteolysis were assessed as a function of time and treatment with
17β-estradiol. 2. Material and methods … Primary antibodies against ERα were from Chi Scientific
(Maynard, MA, #8-80119, #8-80113); antibodies to GAPDH were from Millipore-Sigma (St …
Cited by 1 Related articles All 6 versions
4.Estradiol receptor profile and estrogen responsiveness in laryngeal cancer and clinical outcomes
N Schwartz, A Verma, C Muktipaty, C Bivens… - Steroids, 2019 - Elsevier
… Louis, MO, #C4555-10G) [16]. Estrogen receptors were inhibited using a neutralizing antibody
to ERα36 (Chi Scientific, Maynard, MA) [37]. Confluent cultures were pre-treated with media
containing different concentrations of the inhibitor as indicated below …
Cited by 6 Related articles All 5 versions
5.Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism
RA Chaudhri, A Hadadi, KS Lobachev… - … et Biophysica Acta (BBA …, 2014 - Elsevier
… Estrogen receptors (ERs) play a major role in classification, diagnosis, and treatment of breast
cancer [13], [14], [15] … ERα36 overexpression plasmids were purchased from Chi Scientific
(Maynard, MA). Polyfect transfection reagent was obtained from Qiagen (Germantown, MD) …
6.Membrane estrogen signaling enhances tumorigenesis and metastatic potential of breast cancer cells via estrogen receptor-α36 (ERα36)
RA Chaudhri, R Olivares-Navarrete, N Cuenca… - Journal of Biological …, 2012 - ASBMB
… needed. The purpose of this study was to evaluate the role of ERα36 in membrane-
associated estrogen signaling in breast cancer … Sciences. Polyclonal ERα66 and ERα36
antibodies were purchased from Chi Scientific (Maynard, MA) …
Cited by 90 Related articles All 8 versions
7.Regulation of Mouse Slo Gene Expression MULTIPLE PROMOTERS, TRANSCRIPTION START SITES, AND GENOMIC ACTION OF ESTROGEN
P Kundu, A Alioua, E Stefani, L Toro - Journal of Biological Chemistry, 2007 - ASBMB
… Estrogen-treated MCF7 cell nuclear extract was from Active Motif … of nuclear extract from HeLa
cells transfected with hERα was resolved in a 4-20% gradient SDS gel and immunoblotted using
a polyclonal antibody raised against hERα (ER-α66, CHI Scientific) as described …
Cited by 46 Related articles All 6 versions
相关实验
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
在免疫反应方面，基于抗原的刺激在体内形成、并与抗原作特异性结合的蛋白质之总称。其化学实质是免疫球蛋白。抗体，按照抗原抗体反应的类型可分别称为沉淀素、凝集素、溶血素、溶菌素、抗毒素、中和抗体等，但这些并不等于有相应的不同抗体存在，一般多只表明抗体反应的多样性而已。抗体在血清中含量最多（体液抗体=humoral antibody）；含有某特定抗体的血清称为抗血或免疫血清。
机体在抗原物质刺激下，由B细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白。因为最初有人用电泳证明血清中抗体活性在γ球蛋白部分，故曾把抗体统称为两种（γ）球蛋白。后来证明，抗体并不都在γ区；而且位于γ区的球蛋白，也不一定都具有抗体活性。1964年，世界卫生组织举行专门会议，将具有抗体活性以及与抗体相关的球蛋白统称为免疫球蛋白（Ig）。如骨髓瘤蛋白，巨球蛋白血症、冷球蛋白血症等患者血清中存在的异常免疫球蛋白以及“正常人”天然存在的免疫
技术资料研选
anti-hER-α66 antibody 抗体
￥3284
