- ¥4200
- Frdbio
- NP068576H
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
50ug
纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
Measured its binding ability with S1-RBD by ELISA.
Immobilized 2019-nCoV Spike Protein (RBD, mFc Tag)( Cat No.:nCoV-S) at 2μg/mL (100μL/well) can bind human ACE2 protein (His tag),the EC50 of ACE2 is 20-90ng/mL
蛋白构建
A DNA sequence encoding the human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with a 6×histidine tag at the C-terminus in HEK293 Cells.
表达宿主
HEK293 Cells
种属
Human
蛋白序列（预测）：
10 20 30 40 50 60 QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS 70 80 90 100 110 120 TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN 130 140 150 160 170 180 PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE 190 200 210 220 230 240 DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS 250 260 270 280 290 300 PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS 310 320 330 340 350 360 VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG 370 380 390 400 410 420 HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN 430 440 450 460 470 480 FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY 490 500 510 520 530 540 CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM 550 560 570 580 590 600 LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS 610 620 630 640 650 660 IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRQYFLK VKNQMILFGE EDVRVANLKP 670 680 690 700 710 720 RISFNFFVTA PKNVSDIIPR TEVEKAIRMS RSRINDAFRL NDNSLEFLGI QPTLGPPNQP PVSHHHHHH
分子量
The recombinant human ACE2 consists of 729 amino acids and predicts a molecular mass of 84.4 kDa.
缓冲液
Sterile PBS, pH 7.4.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to 12 months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
Construction of Recombinant Human Cytomegalovirus
genome. These studies can extend to the introduction of viral or foreign genetic material into ectopic sites in the viral genome, to investigate viral cis -control sequences or to phenotypically modify or tag the recombinant virus. Genetically modified
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
The cellular retinaldehyde-binding protein (CRALBP) is expressed at high levels in vertebrate visual trssue, where it may serve to modulate the interaction of 11-cis -retinol with visual-cycle enzymes in the retinal pigment epithelium (RPE
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