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- 2026年01月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 规格：
1mg
纯度：
> 95 % as determined by SDS-PAGE.
蛋白构建:
A DNA sequence encoding the SARS-COV-2（2019-nCoV）nucleocapsid protein was expressed with His-tag in N terminus.
表达宿主：
Escherichia coli
种属：
SARS-COV-2(2019-Ncov)
蛋白序列：
MHHHHHHMSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA.
分子量:
The nucleocapsid protein of 2019-nCoV nucleocapsid consists of 419 amino acids.
缓冲液：
Liquid in sterile PBS, pH7.4.
产品保存与运输:
Recombinant proteins are provided as frozen liquid which are shipped with Blue ice.
Bulk packages can be provided as lyophilized powder which shipped with blue ice.
关于N蛋白:
Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways.
重要提示：
This material is offered by Gene Universal for research, laboratory or further evaluation purposes.
Not for human use.
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- 作者
- 内容
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
-tag fusion protein is in soluble form, this supernatant is ready for L5 column purification. If the DYKDDDDK-tag fusion protein is in an insoluble form, solubilize the inclusion body after centrifugation step (Step 6), refold the protein properly
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
Protein Expression and Purification of RPA70-AB (181-422)
Comments: pSV281 RPA70AB Constructed by L.Mizoue; May '02.This plasmid encodes RPA 70 subunit residues 181-422 as a fusion protein with an NT 6His tag.The tag can be cleaved off with TEV protease (recognition site is ENLFYQG)leaving "GS" at NT
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