GFAP-ICC, WB(Human, Rat)[Sheep

如何做出一手漂亮的 WB 结果？

条件至关重要。1. 转膜时间2. 转膜过程环境温度3. 转膜缓冲液使用次数4．转膜缓冲液中甲醇比例（v/v）（三）一抗使用* 使用 IRS1（150kDa）、pPERK（125kDa）和内质网应激 XBP1（29/55kDa）抗体等检测小鼠肝组织探讨一抗在多种条件下对结果影响（IRS1，pPERK 和 XBP1 用于 WB 检测难以出现清晰条带，因此选择合适的一抗及孵育条件就很关键）。1. 一抗稀释溶剂2. 一抗稀释比例3. 一抗孵育时间（四）二抗使用* 高浓度的二抗会导致大量非特异性结合，造成 WB

Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA

The quantification of human immunoglobulin G (IgG) and recombinant derivatives is required for many applications in research and diagnostics. Particularly in research, robust and simple protocols are necessary to determine the concentration

Production of Transgenic Sheep

The production of transgenic sheep has proven difficult compared to the mouse and lower animals. The work load is far greater and the rates of success far less by most criteria. However, the benefits to human and animal health and agricultural