自备物品 15毫升锥形离心管:用于制备样品的容器 比色皿:用于比色的容器 96孔酶标板:用于比色的容器 分光光度仪:用于比色分析 酶标仪:用于微量样品比色分析 储存条件 14℃或-20℃ 拟态弧菌核酸检测试剂盒(恒温荧光法)规格准备物品 清理液(A) 毫升 染色液(t B) 微升 稀释液(C) 毫升 溶解液(tD) 毫升 产品说明书 1份 操作方法 1直接测定 2测定准备 3背景对照测定 4样品活性测定 5计算样品活性 使用方法: 注:产品仅用于科研所有试剂使用前需完全解冻,混合均匀,6,000rpm离心数秒后使用。 1. 样本处理(样本处理区) 待检样本的核酸提取可采用病毒RNA提取试剂盒或自动化核酸提取仪等,具体提取方法请参照相关说明书;阳性质控品及阴性质控品无需提取,可直接使用。 2. 扩增试剂准备(PCR前准备区) 取N个(N=阴性质控品+待检样本+阳性质控品)PCR反应管,每管分别加入FMD RT-PCR反应液19μl、RT-PCR酶1 μl (也可根据每头份用量计算N+1份FMD RT-PCR反应液、RT-PCR酶所需总量,两者混匀离心后分装20 μl至单个PCR反应管)。 组分每头份用量FMD RT-PCR反应液19 μlRT-PCR酶。 1 .将所有PCR反应管于6,000rpm离心30s,转移至样本处理区。 2.加样(样本处理区) 3.在上述的PCR反应管中分别加入待检样本RNA提取物、阴性质控品和阳性质控品各5 μl,盖紧管盖,于6,000rpm离心10s,转移至扩增区。 其他备注 1.本产品为50次操作 2.操作时,须戴手套 3.操作时,避免污染母液 4.即刻进行荧光检测分析 5.本产品染色剂不易洗掉,染色持久 6.本公司提供系列试剂产品 以下是相关产品: 3-(Trimethylsilyl)propionic Acid-d4 Sodium Salt1076199-49-9规格含量:250mgChemical Name:N-Trifluoroacetyl-4-benzyloxy-3-methoxyphenethylamineSynonyms:N-(4-Benzyloxy-3-methoxyphenethyl)-2,2,2-trifluoroacetamide; 2,2,2-Trifluoro-N-[2-[3-methoxy-4-(phenylmethoxy)phenyl]ethyl]acetamideCAS Number:1076199-49-9Molecular Formula:C₁₈H₁₈F₃NO₃Appearance:Off-White SolidMolecular Weight:353.34Storage:Refrigerator 6-(Tetrahydropyran-4-yloxy)nicotinic Acid252337-58-9规格含量:10mgChemical Name:5-[N(6-(Trifluoroacetamido)hexyl)-3-(E)-acrylamido]-2’-deoxyuridineSynonyms:2'-Deoxy-5-[3-oxo-3-[[6-[(trifluoroacetyl)amino]hexyl]amino]-1-propenyl]-uridine;CAS Number:252337-58-9Molecular Formula:C₂₀H₂₇F₃N₄O₇Molecular Weight:492.45Category:Building Blocks;Applications:5-[N(6-(Trifluoroacetamido)hexyl)-3-(E)-acrylamido]-2’-deoxyuridine is a derivative of 5-[3-(Trifluoroacetamido)-E-1-propenyl]-2’-deoxyuridine (T785010), which can be used as reactant/reagent for "Corey's reagent," 3,5-di-tert-butyl-1,2-benzoquinone, as a modifying agent in the synthesis of fluorescent and double-headed nucleosides. 4-[2-(Trimethylsilyl)ethynyl]pyrene规格含量:25mgChemical Name:6-(2-(Trimethylsilyl)ethoxy Dehydro MaltotetraoseMolecular Formula:C₂₉H₅₄O₂₁SiAppearance:White SolidMolecular Weight:766.81Category:Building Blocks; Carbohydrates; Miscellaneous;Applications:6-(2-(Trimethylsilyl)ethoxy Dehydro Maltotetraose is an intermediate used in the synthesis of Maltotetraose Deuterated (M161522), which is an unlabelled form of Maltotetraose (M161520). Maltotetraose is a maltooligosaccharide that is used for research and diagnostic purposes. They can also be used in nutrients and healthcare. Trimethylsilyl(trifluoromethane)1344922-37-7规格含量:10mgChemical Name:(2S)-3-[(3,3,3-trifluoropropyl)amino]propane-1,2-diolCAS Number:1344922-37-7Molecular Formula:C₆H₁₂NO₂F₃Molecular Weight:187.16Category:Building Blocks; Miscellaneous;Applications:(2S)-3-[(3,3,3-trifluoropropyl)amino]propane-1,2-diol (cas# 1344922-37-7) is a useful research chemical. 2-(Trifluoromethyl)quinoline340-07-8规格含量:5gChemical Name:N-Trifluoroacetyl PiperidineSynonyms:2,2,2-Trifluoro-1-(1-piperidinyl)ethanone; 1-(Trifluoroacetyl)piperidine; 2,2,2-Trifluoro-1-(piperidin-1-yl)ethanone;CAS Number:340-07-8Molecular Formula:C₇H₁₀F₃NOAppearance:Light Yellow OilMolecular Weight:181.16Storage:Refrigerator 2'-(Trifluoromethyl)-[1,1'-biphenyl]-3-carboxylic Acid394-59-2规格含量:2.5gChemical Name:p-TrifluoroacetyltolueneSynonyms:1-Methyl-4-(trifluoroacetyl)benzene; 2,2,2-Trifluoro-1-(4-methylphenyl)ethanone; 2,2,2-Trifluoro-4'-methylacetophenone; 4-Methyl-α,α,α-trifluoroacetophenone; 4-Methylphenyl Trifluoromethyl Ketone; 4'-Methyl-2,2,2-trifluoroacetophenone; NSC 142255; Trifluoromethyl 4-Methylphenyl Ketone; Trifluoromethyl p-Tolyl Ketone; p-Methyl-α,α,α-trifluoroacetophenone; p-Methylphenyl Trifluoromethyl Ketone; α,α,α-Trifluoro-p-methylacetophenoneCAS Number:394-59-2Molecular Formula:C₉H₇F₃OAppearance:Clear Colourless OilMelting Point:N/AMolecular Weight:188.15 3-(Trifluoromethyl)phenylboronic Acid Pinacol Ester182439-11-8规格含量:100mgChemical Name:9-[[(2,2,2-Trifluoroethyl)amino]carbonyl]-9H-fluorene-9-butanoic AcidCAS Number:182439-11-8Molecular Formula:C₂₀H₁₈F₃NO₃Molecular Weight:377.36Category:Standards; Pharmaceutical/API Drug Impurities/Metabolites;Applications:9-[[(2,2,2-Trifluoroethyl)amino]carbonyl]-9H-fluorene-9-butanoic Acid is a Lomitapide metabolite. Lomitapide (L469435) is a drug used in the treatment of obesity and aids in lowering LDL cholesterol and overall cholesterol levels in subjects. 3-(5-(Trifluoromethyl)-1,2,4-oxadiazol-3-yl)benzoic Acid402-49-3规格含量:5gChemical Name:4-(Trifluoromethyl)benzyl BromideSynonyms:α'-Bromo-α,α,α-trifluoro-p-xylene; 1-(Bromomethyl)-4-(trifluoromethyl)benzene; 1-Trifluoromethyl-4-(bromomethyl)benzene; 4-Bromomethylbenzotrifluoride; p-(Trifluoromethyl)benzyl bromide; α'-Bromo-α,α,α-trifluoro-p-xyleneCAS Number:402-49-3Molecular Formula:C₈H₆BrF₃Molecular Weight:239.03Category:Building Blocks; Miscellaneous;Applications:4-(Trifluoromethyl)benzyl Bromide is a useful building block. It is used in the synthesis of 1-(Substituted benzyl)-2-substituted-5,6-dichlorobenzimidazoles with antiviral activities.It is also used to prepare 2-[(4-diarylmethoxy)phenyl]benzimidazoles as potent inhibitors of hepatitis C virus NS5B polymerase. 拟态弧菌核酸检测试剂盒(恒温荧光法)规格TmR2(GDNFR Beta;TRN-R;GFR-alpha-2;NTNR-2) 胶质细胞系源性神经营养因子(多肽) 规格: 0.5mg 特价供应 TNF-Alpha(Tumor Necrosis Factor-Alpha) 人肿瘤坏死因子-α(多肽抗原) 规格: 0.5mg 特价供应 TRA16 (Testicular orphan nuclear receptor-4 (TR4)-associated protein) 激素受体相关蛋白 规格: 0.5mg 特价供应 Tranthyretin 甲状腺素运载蛋白(抗原) 规格: 0.5mg 特价供应 TrK A (h-NGFR) (p140- TrK A) 神经生长因子的一种(抗原) 规格: 0.5mg 特价供应 TrK A.B.C 神经生长因子的一种(抗原) 规格: 0.5mg 特价供应 active Caspase 3(caspase-3 p12 subunit) 活化半胱胺酸蛋白酶蛋白-3抗原 规格: 0.5mg 特价供应 CASP4(Caspase-4) 半胱胺酸蛋白酶蛋白-4抗原 规格: 0.5mg 特价供应 Trk B 神经生长因子受体的一种(抗原) 规格: 0.5mg 特价供应 Trk B 神经生长因子受体的一种(抗原) 规格: 0.5mg 特价供应 兔抗亲和素纯化抗体 特价促销 1mg/10mg 国产 YES1 英文名称: 原癌基因酪酸蛋白激酶Yes1抗体 特价促销 0.1ml Rhesus antibody Rh phospho-c-Raf(Ser339) 磷酸化原癌基因c-Raf抗体 特价促销 规格 0.1ml HA tag HA tag标签多肽Multi-class antibodies规格: 0.5mg DRD4 英文名称: 多巴胺受体D4抗体 特价促销 0.2ml TFRC Transferrin Receptor / TFRC / CD71 兔单抗 (PE) Human