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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide surrounding phospho Ser536 of human NFkB p65. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX133899
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
0.59 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点:
NFkB p65 (phospho Ser536)
- 抗体英文名:
NFkB p65 (phospho Ser536) antibody
- 抗体名:
NFkB p65 (phospho Ser536) 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NFkB p65 (phospho Ser536) antibody detects NFkB p65 (phospho Ser536) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: NFkB p65 (phospho Ser536) stained by NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
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文献和实验Chou LY et al., Cells 2022 (PMID:36291189)
Xue Zhang et al., Front Pharmacol 2022 (PMID:35308231)
Chang JW et al., Cereb Cortex 2020 (PMID:32248223)
Chao CC et al., Cancers (Basel) 2020 (PMID:32079335)
Li CJ et al., J Hepatocell Carcinoma 2023 (PMID:36741246)
Li Y et al., Exp Ther Med 2022 (PMID:35978933)
Alhasan H et al., Nat Commun 2022 (PMID:35798730)
Zhang W et al., Oxid Med Cell Longev 2023 (PMID:36691640)
【求助】于NF-KB 激活,P65磷酸化位点是ser536还是ser276?
激活。所以蛋白的磷酸化或去磷酸化不是简单的孤立现象,它始终与细胞信号转导有关系。最终将达到细胞增殖、分化、激活等功能的实现。 magichunter 楼上回答的是什么啊?人家问的是:于NF-KB 激活,P65磷酸化位点是ser536还是ser276? 你也没回答到底是哪个啊? 伽利略 chenlydd wrote: 我认为蛋白的磷酸化与其功能有关系。蛋白可被磷酸化,也可被去磷
sakura042 本新手欲研究nfkb引起内皮细胞凋亡的机制:是否通过jnk通路。 这是否是一个信号通路串话的问题? 我们用特异性抑制剂阻断nfkb后发现jnk活性下降,是否就能说明nfkb促进内皮细胞凋亡是通过jnk通路呢?还需要再阻断或者过表达jnk通路来反证吗? 这个问题困扰了我好久,请教各位高手。 sakura042 自己顶一下 三叶虫 请问一下
【求助】矛盾的实验结果:IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达,结合文献,推理应该是IKb alpha下降,而NFKB升高 (IKb可磷酸化NFKB,使后者降低) 但现在我的结果是两者同时升高,请问可能的原因有哪些呢? 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况? erik NFKB是检测核内的吗?检测p65还是p50/ 吴佰霖 检测总蛋白
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