
timesaver SDS-PAGE Staining Bu
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- WBK202
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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 保质期:
2年
- 保存条件:
常温
- 规格:
500ml
简单:无需加热,几乎无背景,无需脱色。极速:使蛋白快速染色,2min可以显示明显条带。
灵敏度高:检测下限低至5ng;微量蛋白可适当延长染色时间。安全:不含甲醇,醋酸等有害成分。
经济:可重复使用两至三次。
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文献和实验will give different intensity of the staining. Hence for some protein, the detection limit may be as low as 1 ng. The procedure described below is essentially that reported by Morrissey (Anal. Biochem., 117: 307-310, 1981) and is suitable for sds-page of proteins
Silver Staining of SDS-PAGE Gels(Ⅰ)
1.Rinse gel on glass plate briefly with distilled water. 2.Wash gel in fresh 50% ETOH-12% HAc,with shaking,3 times,1 hour each.(Fix IEF gel ON in 10% TCA). 3.Wash gel four times with 10% ETOH-5% HAc for one hour each.One wash may be done overnight
. 0.1% SDS. Keep a squirt bottle of it.VIII. Running buffer: Keep a jug of this. 24 g Tris base, 115.2 g glycine, 20 ml 20% SDS, H2O to 4 liters.IX. Sample buffer: 100 mM Tris, pH 6.8, 2% SDS, 5% ß- mercaptoethanol, 15% glycerol, enough bromophenol blue
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