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AffiniPure F(ab')2 Fragment Ra

bbit Anti-Goat IgG, F(ab')2 Fragment Specific (min X Hu Sr Prot)
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  • 询价
  • Jackson ImmunoResearch已认证
  • US
  • 305-006-047
  • 2025年12月02日
  • Rabbit
  • Goat
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.

    • 亚型

      IgG, F(ab')2 fragment

    • 形态

      Sterile-filtered liquid

    • 保存条件

      Store at 2-8°C under sterile conditions.

    • 克隆性

      单克隆

    • 标记物

      Unconjugated

    • 适应物种

      Goat

    • 保质期

      One year from date of receipt.

    • 抗原来源

      IgG, F(ab')2 fragment

    • 供应商

      Jackson ImmunoResearch Laboratories Inc.

    • 宿主

      Rabbit

    • 抗体英文名

      AffiniPure F(ab')2 Fragment Rabbit Anti-Goat IgG, F(ab')2 Fragment Specific (min X Hu Sr Prot)

    • 抗体名

      AffiniPure F(ab')2 Fragment

    F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against the Fc portion of goat IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

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    图标文献和实验
    相关实验
    • Jackson ImmunoResearch 二抗选用指南

      -conjugated | AffiniPure | F(ab)2 fragment | of Goat | Anti-Mouse | IgG, F(ab)2 fragment specific

    • 激光共聚焦实验服务案例介绍

      XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂:PBS(磷酸盐缓冲液pH7.4)(实验室常规配置);非免疫山羊血清;Triton X-100(sigma);BSA抗体稀释液(实验室常规配置);A蛋白抗体(Santa cruz);B蛋白抗体(Abcam);Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen);Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab

    • 流式细胞方案

      细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔:  Anti-Rabbit IgG(H+L), Fab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412  Anti-Rabbit IgG(H+L), Fab

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