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文献和实验的RNAiso Reagent量偏少。 ② 使用的组织材料中含有大量的有机溶剂(如:乙醇、异丙醇等)、高浓度的Buffer、碱性溶剂等。 ③ 如果提取的RNA中含有DNA时,可以使用DNase I (RNase Free;TaKaRa Code:D2215)进行DNA消化。 ■ 参考文献 1. J. Chirgwin et al, "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched
实验步骤 1. Routine Protocol The following protocol is optimized for the control DNA and the primers provided with this kit. This protocol may serve as a starting point for any PCR amplification. Critical parameters
Food-Grade Corynebacteria for Enzyme Production
The expression of genes coding for heterologous extracellular enzymes or proteins in corynebacteria has provided new capacities to these industrially important microorganisms, such as the use of the culture media as sources of essential amino
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