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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10个
- 英文名：
Lipofectamine™ 3000 Transfection Reagent
- 保质期：
1年
- 供应商：
朗智（上海）生物科技有限公司
- 保存条件：
2-8°C
- 规格：
0.75ml/1.5ml
| 规格： | 0.75ml | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 1.5ml | 产品价格： | ¥2.0 |
Description
Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection
Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
| Fibroblast (3T3, COS-7) | Myoblast (C2C12, L6 CRL-1458) |
| Hepatocyte (HepG2, HuH7) | Erythroleukemic cell (K562) |
| Breast carcinoma (MCF7, Hs578T) | Prostate carcinoma (LNCap) |
| Lung carcinoma (A549, NCI-H460) | Osteosarcoma (U-2 OS, Saos-2) |
| Colon carcinoma (Caco2, SW480) | Pancreatic carcinoma (PANC-1) |
| Skin melanoma (SK-MEL-28) |
Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).
Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.
Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
Specifications
| Cell Type: | Established Cell Lines,Hard-to-Transfect Cells,Primary Cells,Stem Cells |
|---|---|
| Classification: | Animal Origin-Free |
| Green Features: | Sustainable packaging |
| Product Line: | Lipofectamine® |
| Product Size: | 1.5 mL |
| Sample Type (Specific): | Plasmid DNA,RNAi Plasmids (shRNA, miR),Synthetic siRNA |
| Serum Compatible: | Yes |
| Transfection Technique: | Lipid-Based Transfection |
Contents & storage
Contains one vial of Lipofectamine® 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.更多详情请联系：
朗智（上海）生物科技有限公司
http://lzbiotech.com
Tel: +(86)021-54019192
Mobile: +(86) 13564617637（微信同号）
QQ：370323648
Email: songxinwei@lzbiotech.com
地址: 上海市徐汇区中山南二路777弄1号楼2405室
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文献和实验Multiple Tissue Northern (MTN™)Blots
人、小鼠和大鼠的多种组织来源的高质量mRNA,经电泳分离后预转于尼龙膜上预制的即用型Northern杂交膜，免去RNA电泳操作过程 可检测范围：0.5-10kb 可重复使用 提供ExpressHybTM快速杂交液样品 可靠的优良品质 从1991年始，公司就向广大的生命科学研究者提供高质量的预制杂交膜。而MTN™膜更是在国际著名的学术期刊上有将近3000篇文献引用。 严谨的制作过程 1.由人、小鼠和大鼠的组织经Oligo(dT)-Cellulose 多次层析获取mRNA
Transfection of Mammalian Cells Using Lipofectamine
basal medium Solution B: For each transfection, dilute 12 µl LIPOFECTAMINE Reagent in 375 µl serum-free medium 4. Combine the two solutions, mix gently, and incubate at room temperature for 15-45 min. The solution may appear cloudy
BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
to mammalian cells. Follow the guidelines below when transfecting the BLOCK-iT™ Fluorescent Oligo. Tip: For highly efficient transfection of a wide variety of mammalian cells, we recommend using Lipofectamine™ 2000 Reagent (Catalog no. 11668-027
