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1 kit (96 well×1 test)
formed by glycation with glyoxal on a collagen coating plate.
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文献和实验Collagen Gel Contraction Assay
of a complex infrastructure that includes collagen and other molecules. The manner in which these two components interact is not certain, but the use of recent model systems has provided insights into these processes. The collagen lattice contraction assay
were then diluted to OD260 2 with ChIP dilution buffer (). 60 ul protein A-agarose beads (Upstate Catalog # 16-157) was added to sonicated lysates and rotated at 4 for one hour to reduce the non-specific dinding. 7, Pellet agarose by brief
specific assay of these events very challenging. In this chapter, those particular features of collagen biochemistry which contribute to the difficulty of measuring its degradation will be outlined and the principles of recently developed methodology
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