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运用:电镜,ICC(1:2000),IP, WB(1:4000)。
浓度:1mg/mL。
特异性:识别蛋白翻译后修饰聚谷氨酸化。与聚谷氨酸化的alpha或者beta-微管蛋白反应。
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文献和实验Proteolytic Fragmentation for Epitope Mapping
the epitope on the antigen recognized by a given MAb is outlined. We describe our studies (2 ) with the transducin (Gt ) α-subunit in which proteolytic digestion, SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, and Edman degradation
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
antibody (mAb) conjugated with peroxidase (anti-DNA-POD) was used. This anti-DNA-POD mAb binds to single- and double-stranded, low molecular weight DNA fragments (mono- and oligonucleosomes), showing the internucleosomal degradation of genomic DNA occuring
14 Immunotargeting of Catalase to the Pulmonary Vascular Endothelium
(anti-ACE MAb 9B9, produced by Dr. Sergei M. Danilov [9 ]), accumulates in the pulmonary endothelium after systemic injection (10 ). Therefore, MAb 9B9 may serve as an affinity carrier for targeting of catalase to the pulmonary endothelial cells
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