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文献和实验Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation
of human platelets. Such an approach offers the possibility of capturing the dynamic tyrosine phosphorylation events that occur upon platelet activation and aggregation, with an aim to identify novel signaling proteins.
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
tube of platelets.PROCEDURE for Flow Cytometric Analysis-Human Platelets 1. Add 100 μL of platelet suspension to the bottom of each tube.2. Add the appropriate antibody to each tube.3. Shake gently and incubate in the dark at RT for 20-30 minutes
Lectin-Induced Calcium Mobilization in Human Platelets: Use of Fluorescent Probes
The cytosolic calcium concentration, (Ca2+ )c , represents in many types of cells a versatile regulatory system involved in several signal transduction pathways of cell activation (1 ), and is tightly regulated in human platelets
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