FluoForte® Calcium assay kit f

Homogeneous mix-and-read, no-wash calcium mobilization assay Load dye in live cells at 37°C or even room temperature for easier automation Broadly applicable to both GPCR and calcium ion channel targets Significantly brighter fluorescence intensity than competitors’ no-wash kits Provides comparable EC50 values as obtained using Fluo-3, Fluo-4 and FLIPR® Calcium 4 assay kits No quencher dye, so no potential interference from compound ligand-receptor interactions Larger assay window than other dyes, allows measurements of challenging cell lines and receptors FluoForte® Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, FluoForte® dye yields the brightest signal and largest assay window. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines. In addition, it requires neither a washing step, nor exogenous addition of a quencher dye, which could adversely effect receptor-ligand interaction kinetics.FluoForte® dye offers superior measurement of challenging cell lines and receptors, unlike other widely used calcium ion indicators in high-throughput screening applications; Fluo-3, Fluo-4 and Calcium 4 dyes have relatively weak fluorescence signals limiting their application in these instances.