Anti-phospho-ATM (Tyr170)/FITC

抗体制备过程：
1.材料与试剂
  a．提取的动物 Ig
  b．弗氏完全佐剂和弗氏不完全佐剂
  c．青霉素和链霉素
  d．实验动物 兔
  e．其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试，查看免疫效果。
5、如果免疫成功，杀死实验活体，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清（无菌采制）

产品名称	Anti-phospho-ATM (Tyr170)/FITC抗体
英文名称	Anti-phospho-ATM (Tyr170)/FITC
货号	XGK3475

英文名称  Anti-phospho-ATM (Tyr170)/FITC
中文名称  FITC标记的磷酸化毛细血管扩张性共济失调症突变蛋白抗体 
别    名ATM (Phospho-Tyr170); ATM (Phospho Y170); p-ATM (Tyr170); p-ATM (Y170); A-T mutated; A-T mutated homolog; AT complementation group A; AT complementation group C; AT complementation group D; AT complementation group E; AT mutated; AT protein; AT1; ATA; Ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated gene; Ataxia telangiectasia mutated homolog (human); Ataxia telangiectasia mutated homolog; ATC; ATD; ATDC; ATE; ATM; ATM_HUMAN; DKFZp781A0353; Human phosphatidylinositol 3 kinase homolog; MGC74674; Serine protein kinase ATM; Serine-protein kinase ATM; Serine/threonine-protein kinase ATM; T cell prolymphocytic leukemia; Tefu; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; Telomere fusion protein; TPLL.
规格:100ul
说 明 书100ul
产品类型磷酸化抗体
研究领域细胞生物  信号转导  细胞凋亡  细胞周期蛋白  激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
产品应用ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量370kDa
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human ATM around the phosphorylation site of Tyr170 [SV(p-Y)FR]
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍background:
ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases)
Function:
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
Subcellular Location:
Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
Tissue Specificity:
Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
我司提供以下技术外包服务:
1. Anti-phospho-ATM (Tyr170)/FITC抗体分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等
2.蛋白工程：原核、哺乳动物蛋白表达系统等
3.病毒包装：腺病毒、慢病毒等
4.抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
5.细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。
抗体的鉴定：
1）抗体的效价鉴定：鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
3）真核翻译起始因子2C2抗体的亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。
小鼠V-Ha-Ras肉瘤病毒癌基因同源物(HRAS)ELISA试剂盒 ，英文名： HRAS ELISA Kit
大鼠血小板衍生生长因子(PDGF)ELISA检测试剂盒Ratplatelet-derivedgrowthfactor,PDGFELISAKit 96T/48T
人HSP60 IgG抗体免疫试剂盒 Human Hsp60 IgG aibody ELISA kit
英文名称MousebFGFELISAKit小鼠碱性成纤维生长因子(bFGF)规格：96T/48T
冰冻切片半胱氨酸蛋白酶-7(CASPASE-7)活性原位荧光染色检测试剂盒50次
Rat8-isoprostaglandin,8-iso-PGELISA试剂盒大鼠8异前列腺素(8-iso-PG)ELISA试剂盒规格：96T/48T
人骨碱性0酸酶(BALP)ELISA 试剂盒 96T/48T 试剂盒 组装/原装
小鼠白三烯D4(LTD4)免疫试剂盒 Mouse leukoiene D4,LT-D4 ELISA Kit
信号传导子及转录激活子4(STAT4)ELISA试剂盒 ，英文名： STAT4 ELISA Kit
MousehepatitisBviruseaigen,HBeAgELISA试剂盒小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒规格：96T/48T
ELISAKiBP-4兔子视黄醇结合蛋白4规格：48T/96T
Chickenbasicfibroblastgrowthfactor,bFGFELISAKit鸡碱性成纤维细胞生长因子(bFGF)ELISA试剂盒规格：96T/48T
人可溶性0脂酶A2(sPL-A2)ELISA 试剂盒 96T/48T 试剂盒 组装/原装
小鼠尿激酶型纤溶酶原激活物(uPA)免疫试剂盒 Mouse urokinase plasminogen activator,uPA ELISA kit
坏死因子受体超家族成员18(TNFRSF18)ELISA试剂盒 ，英文名： TNFRSF18 ELISA Kit
Humaumournecrosisfactorrelatedweakinducerofapoptosis,TWEAKELISA试剂盒人坏死因子相关弱凋亡诱导因子(TWEAK)ELISA试剂盒规格：96T/48T
ELISAKitsP-selectin小鼠可溶性P选择素规格：48T/96T
Humanai-hepatitisBviruscoreaibody，HBcAbELISAKit人抗乙型肝炎病毒核心抗体(HBcAb)ELISA试剂盒规格：96T/48T
猪胚胎传代细胞;ST
SELPLG Others Mouse 小鼠 PSGL-1 / CD162 / SELPLG 人细胞裂解液 (阳性对照)
Huh-7 人肝癌细胞
NCI-H2452 [H2452]人间皮瘤细胞 NCI-H2452 [H2452] human skin tumor cells RPMI-1640(GIBCO)+10%FBS
PGF Protein Mouse 重组小鼠 PIGF / PLGF 蛋白 (Fc 标签)
马间叶干细胞(脂肪)(5×105) Raw264.7, 小鼠单核巨噬细胞白血病细胞 Mouse
Anti-phospho-ATM (Tyr170)/FITC抗体MMQ 小鼠垂体瘤细胞
HUVEC-12, 人脐静脉血管内皮细胞系
人表皮淋巴内管皮细胞(HDLEC)(5×105)
人Burkkit淋巴瘤细胞;Daudi 大鼠肾小球内皮细胞完全培养基 100mL
人非小细胞肺腺癌细胞;NCI-H2087
FETUB Others Human 人 Fetuin-B / FETUB 人细胞裂解液 (阳性对照)