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Recombinant Human SLAMF1 Sf9
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文献和实验Native purification of His-tagged Proteins from Sf9 cells
Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0 1 % NP-40 1 mM PMSF 1 µg/ml leupeptin 3. Transfer to oakridge tubes and sonicate
GPCR Expression Using Baculovirus-Infected Sf9 Cells
Expression of proteins in insect cells using recombinant baculoviruses has gained wide use in the G protein-coupled receptor (GPCR) community. This expression system produces high yields of functional receptor, is able to perform post
Purification of Recombinant p53 from Sf9 Insect Cells
We describe a method for purifying recombinant p53 from baculovirus infected cells in one step by anion exchange chromatography. The p53 is full-length with no flanking sequences and its expression is driven by the baculovirus polyhedron
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