AITRL, T7

T7 siRNA protocol

Here is our T7 siRNA protocol. The main idea is to design primers that begin with GG so thattranscription by T7 polymerase is efficient.I) FIRST design oligos:Using sense coding sequence of any gene....(N1, N2, N22, N23, are numbered positions

Cesium Chloride Purification of T7

  Summary Cleaner stocks of T7 that concentrates and purifies T7 bacteriophage. Protocol Grow 100mL of permissive cells to a density of 108 to 109 cells/ml at 30°C in a rotary shaking water bath. Inoculate the cells

mRNA Amplification with T7 RNA Polymerase

on a 42ºC oven and set PCR machine to hold at 70ºC. In an Eppendorf tube add: 1 µg Total RNA 1 µL T7 oligo(dT) primer q.s. to 12 µL with Nuclease-free H2 O Incubate 10 min. at 70ºC.  Spin briefly to pull