- 询价
- 西格
- XGK1415
- 进口、国产
- 2025年07月14日
- WB=1:100-500 ELISA=1:500-1000 IF=1:100-500
- 详见说明书
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海西格
- 库存：
28
- 靶点：
详见说明书
- 级别：
详见说明书
- 目录编号：
详见说明书
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 抗体英文名：
Anti-ER-Alpha
- 抗体名：
雌激素受体α抗体（用于western blot）
- 标记物：
详见说明书
- 宿主：
详见说明书
- 适应物种：
详见说明书
- 免疫原：
KLH conjugated synthetic peptide derived from human ER-Alpha C-terminus
- 亚型：
IgG
- 形态：
详见说明书
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IF=1:100-500
- 浓度：
1mg/1ml
- 保存条件：
详见说明书
- 规格：
0.1ml/100μg 0.2ml/200μg
实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
英文名称 Anti-ER-Alpha
中文名称 雌激素受体α抗体（用于western blot）
别 名 Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1 (alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human
产品类型 一抗
研究领域 肿瘤 染色质和核信号 信号转导 内分泌病 肿瘤细胞生物标志物 表观遗传学
蛋白分子量 predicted molecular weight: 66kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human ER-Alpha C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.
Function : Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1.
Subunit : Binds DNA as a homodimer. Can form a heterodimer with ESR2. Isoform 3 can probably homodimerize or heterodimerize with isoform 1 and ESR2. Interacts with FOXC2, MAP1S, SLC30A9, UBE1C and NCOA3 coactivator (By similarity). Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent. Interacts with NCOA5 and NCOA6 coactivators. Interacts with NCOA7; the interaction is a ligand-inducible. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via C-terminus). Interacts with MACROD1. Interacts with SH2D4A and PLCG. Interaction with SH2D4A blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PI3KR1 or PI3KR2, SRC and PTK2/FAK1. Interacts with RBFOX2. Interacts with STK3/MST2 only in the presence of SAV1 and vice-versa. Binds to CSNK1D. Interacts with NCOA2; NCOA2 can interact with ESE1 AF-1 and AF-2 domains simultaneously and mediate their transcriptional synergy. Interacts with DDX5. Interacts with NCOA1; the interaction seems to require a self-association of N-terminal and C-terminal regions. Interacts with ZNF366, DDX17, NFKB1, RELA, SP1 and SP3. Interacts with NRIP1 (By similarity).
Subcellular Location : Isoform 1: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane.
Isoform 3: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Single-pass type I membrane protein. Note=Associated with the inner membrane via palmitoylation (Probable). At least a subset exists as a transmembrane protein with a N-terminal extracellular domain.
Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated.
Tissue Specificity : Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Post-translational modifications : Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
抗体制备过程：
1.材料与试剂
a．提取的动物 Ig
b．弗氏完全佐剂和弗氏不完全佐剂
c．青霉素和链霉素
d．实验动物 兔
e．其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试，查看免疫效果。
5、如果免疫成功，杀死实验活体，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清（无菌采制）
我司提供以下技术外包服务:
1. Anti-ER-Alpha抗体分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等
2.蛋白工程：原核、哺乳动物蛋白表达系统等
3.病毒包装：腺病毒、慢病毒等
4.抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
5.细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。
Anti-TPO/FITC 荧光素标记兔抗甲状腺过氧化物酶抗体IgGMulti-class antibodies规格： 0.2ml
PP-2B alpha 1 蛋酸酯酶-2B 催化亚单位(抗原)Multi-class antibodies规格： 0.5mg
抗甲状腺过氧化物酶抗体 Anti-TPO 0.1ml
AP180 英文名称： 装配衔接蛋白180抗体 0.2ml
EGR3 英文名称： 早期生长反应蛋白3抗体 0.1ml
Rhesus antibody Rh phospho-ITGB4(Tyr1530) 0酸化整合素β4抗体 规格 0.1ml
PP-2B alpha 1 蛋酸酯酶-2B 催化亚单位(抗原)Multi-class antibodies规格： 0.5mg
pre-X protein[Hepatitis B virus]N-Terminus 乙肝病毒pre-X蛋白抗原(N端)Multi-class antibodies规格： 0.5mg
Anti-Aurora A/B/C 有丝分裂激酶A/B/C抗体Multi-class antibodies规格： 0.2ml
Rhesus antibody Rh phospho-Akt(Thr308) 0酸化蛋白激酶B抗体 规格 0.1ml
MBP 浓缩液 0.1ml 进口分装
UNC93B 英文名称： 内质网膜蛋白UNC93B抗体 0.2ml
Choline kinase alpha 英文名称： 乙激酶α抗体 0.1ml
Anti-Aurora A/B/C 有丝分裂激酶A/B/C抗体Multi-class antibodies规格： 0.2ml
S100A11 英文名称： S100钙结合蛋白A11抗体 0.1ml
EpCAM 英文名称： 上皮特异性抗原抗体 0.1ml
信号转导和转录激活因子2抗体 Anti-STAT2 0.2ml
anti-Rarres2/TIG2/FITC 荧光素标记视黄酸受体应答蛋白2抗体IgGMulti-class antibodies规格： 0.2ml
Rhesus antibody Rh Phospho-MAP2 (Thr1620/1623) 0酸化微管相关蛋白2抗体 规格 0.1ml
Nociceptin receptor 孤菲肽受体(痛敏肽受体)(抗原)Multi-class antibodies规格： 0.5mg
小鼠Ⅲ型胶原交联羧基端肽(CTXⅢ)ELISA试剂盒 ，英文名： CTXⅢ ELISA Kit
人孕酮受体(PGR)ELISA检测试剂盒Humanprogesteronereceptor,PGRELISAKit 96T/48T
大鼠防御素5 免疫试剂盒 Rat defensin-5 ELISA kit
CLIAKitforUKELISAKit大鼠尿激酶规格：48T/96T
尿素氮定量检测试剂盒50次
sAIL人可溶性坏死因子相关凋亡诱导配体规格：48T/96T
Anti-ER-Alpha抗体人基转移酶(ALT)ELISA试剂盒 96T/48T 试剂盒 组装/原装
豚鼠白介素10(IL-10)免疫试剂盒 Guinea pig ierleukin 10,IL-10 ELISA Kit
酸性成纤维细胞生长因子(aFGF)ELISA试剂盒 ，英文名： aFGF ELISA Kit
Mouseα-HydroxybyrateDehydrogenase,αHBDHELISA试剂盒小鼠α羟基脱氢酶(αHBDH)ELISA试剂盒规格：96T/48T
GoatIerleukin4,IL-4ELISA试剂盒山羊白介素4(IL-4)ELISA试剂盒规格：96T/48T
Humanai-braiissueaibody,ABAbELISAKit人抗脑组织抗体(ABAb)ELISA试剂盒规格：96T/48T
抗体的鉴定：
1）抗体的效价鉴定：鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
3）真核翻译起始因子2C2抗体的亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
| 产品名称 | 雌激素受体α抗体（用于western blot） |
| 英文名称 | Anti-ER-Alpha抗体 |
| 货号 | XGK1415 |
中文名称 雌激素受体α抗体（用于western blot）
别 名 Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1 (alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human
产品类型 一抗
研究领域 肿瘤 染色质和核信号 信号转导 内分泌病 肿瘤细胞生物标志物 表观遗传学
蛋白分子量 predicted molecular weight: 66kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human ER-Alpha C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.
Function : Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1.
Subunit : Binds DNA as a homodimer. Can form a heterodimer with ESR2. Isoform 3 can probably homodimerize or heterodimerize with isoform 1 and ESR2. Interacts with FOXC2, MAP1S, SLC30A9, UBE1C and NCOA3 coactivator (By similarity). Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent. Interacts with NCOA5 and NCOA6 coactivators. Interacts with NCOA7; the interaction is a ligand-inducible. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via C-terminus). Interacts with MACROD1. Interacts with SH2D4A and PLCG. Interaction with SH2D4A blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PI3KR1 or PI3KR2, SRC and PTK2/FAK1. Interacts with RBFOX2. Interacts with STK3/MST2 only in the presence of SAV1 and vice-versa. Binds to CSNK1D. Interacts with NCOA2; NCOA2 can interact with ESE1 AF-1 and AF-2 domains simultaneously and mediate their transcriptional synergy. Interacts with DDX5. Interacts with NCOA1; the interaction seems to require a self-association of N-terminal and C-terminal regions. Interacts with ZNF366, DDX17, NFKB1, RELA, SP1 and SP3. Interacts with NRIP1 (By similarity).
Subcellular Location : Isoform 1: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane.
Isoform 3: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Single-pass type I membrane protein. Note=Associated with the inner membrane via palmitoylation (Probable). At least a subset exists as a transmembrane protein with a N-terminal extracellular domain.
Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated.
Tissue Specificity : Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Post-translational modifications : Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
抗体制备过程：
1.材料与试剂
a．提取的动物 Ig
b．弗氏完全佐剂和弗氏不完全佐剂
c．青霉素和链霉素
d．实验动物 兔
e．其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试，查看免疫效果。
5、如果免疫成功，杀死实验活体，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清（无菌采制）
我司提供以下技术外包服务:
1. Anti-ER-Alpha抗体分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等
2.蛋白工程：原核、哺乳动物蛋白表达系统等
3.病毒包装：腺病毒、慢病毒等
4.抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
5.细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。
Anti-TPO/FITC 荧光素标记兔抗甲状腺过氧化物酶抗体IgGMulti-class antibodies规格： 0.2ml
PP-2B alpha 1 蛋酸酯酶-2B 催化亚单位(抗原)Multi-class antibodies规格： 0.5mg
抗甲状腺过氧化物酶抗体 Anti-TPO 0.1ml
AP180 英文名称： 装配衔接蛋白180抗体 0.2ml
EGR3 英文名称： 早期生长反应蛋白3抗体 0.1ml
Rhesus antibody Rh phospho-ITGB4(Tyr1530) 0酸化整合素β4抗体 规格 0.1ml
PP-2B alpha 1 蛋酸酯酶-2B 催化亚单位(抗原)Multi-class antibodies规格： 0.5mg
pre-X protein[Hepatitis B virus]N-Terminus 乙肝病毒pre-X蛋白抗原(N端)Multi-class antibodies规格： 0.5mg
Anti-Aurora A/B/C 有丝分裂激酶A/B/C抗体Multi-class antibodies规格： 0.2ml
Rhesus antibody Rh phospho-Akt(Thr308) 0酸化蛋白激酶B抗体 规格 0.1ml
MBP 浓缩液 0.1ml 进口分装
UNC93B 英文名称： 内质网膜蛋白UNC93B抗体 0.2ml
Choline kinase alpha 英文名称： 乙激酶α抗体 0.1ml
Anti-Aurora A/B/C 有丝分裂激酶A/B/C抗体Multi-class antibodies规格： 0.2ml
S100A11 英文名称： S100钙结合蛋白A11抗体 0.1ml
EpCAM 英文名称： 上皮特异性抗原抗体 0.1ml
信号转导和转录激活因子2抗体 Anti-STAT2 0.2ml
anti-Rarres2/TIG2/FITC 荧光素标记视黄酸受体应答蛋白2抗体IgGMulti-class antibodies规格： 0.2ml
Rhesus antibody Rh Phospho-MAP2 (Thr1620/1623) 0酸化微管相关蛋白2抗体 规格 0.1ml
Nociceptin receptor 孤菲肽受体(痛敏肽受体)(抗原)Multi-class antibodies规格： 0.5mg
小鼠Ⅲ型胶原交联羧基端肽(CTXⅢ)ELISA试剂盒 ，英文名： CTXⅢ ELISA Kit
人孕酮受体(PGR)ELISA检测试剂盒Humanprogesteronereceptor,PGRELISAKit 96T/48T
大鼠防御素5 免疫试剂盒 Rat defensin-5 ELISA kit
CLIAKitforUKELISAKit大鼠尿激酶规格：48T/96T
尿素氮定量检测试剂盒50次
sAIL人可溶性坏死因子相关凋亡诱导配体规格：48T/96T
Anti-ER-Alpha抗体人基转移酶(ALT)ELISA试剂盒 96T/48T 试剂盒 组装/原装
豚鼠白介素10(IL-10)免疫试剂盒 Guinea pig ierleukin 10,IL-10 ELISA Kit
酸性成纤维细胞生长因子(aFGF)ELISA试剂盒 ，英文名： aFGF ELISA Kit
Mouseα-HydroxybyrateDehydrogenase,αHBDHELISA试剂盒小鼠α羟基脱氢酶(αHBDH)ELISA试剂盒规格：96T/48T
GoatIerleukin4,IL-4ELISA试剂盒山羊白介素4(IL-4)ELISA试剂盒规格：96T/48T
Humanai-braiissueaibody,ABAbELISAKit人抗脑组织抗体(ABAb)ELISA试剂盒规格：96T/48T
抗体的鉴定：
1）抗体的效价鉴定：鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
3）真核翻译起始因子2C2抗体的亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。
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抗核抗体(anti-nucleic antibody，ANA)检测
抗核抗体(anti-nucleic antibody，ANA)是以真核细胞的核成分为靶抗原的自身抗体的总称。细胞核内含有许多不同成分如核蛋白、核糖核蛋白(ribonucleo protein，RNP)、脱氧核糖核酸(DNA)等。 在某些因素，如细菌、病毒、药物等作用下，可改变细胞核某些成分的性质，激发机体免疫系统产生许多抗不同核成分的抗体。
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Anti-ER-Alpha抗体
询价
