- ￥205 - 2795
- 碧云天（beyotime）
- P5084
- 2025年12月22日
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- 规格：
1mg/5mg/20mg/100mg
| 规格： | 1mg | 产品价格： | ￥205.0 |
|---|---|---|---|
| 规格： | 5mg | 产品价格： | ￥516.0 |
| 规格： | 20mg | 产品价格： | ￥1035.0 |
| 规格： | 100mg | 产品价格： | ￥2795.0 |
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P5084-1mg | Recombinant Streptavidin | 1mg | 205.00元 |
| P5084-5mg | Recombinant Streptavidin | 5mg | 516.00元 |
| P5084-20mg | Recombinant Streptavidin | 20mg | 1035.00元 |
| P5084-100mg | Recombinant Streptavidin | 100mg | 2795.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Streptomyces avidinii | X03591 | P22629 | E. coli | 128aa | 52kDa (tetramer) | － |
| About this Protein | |
| Name | Recombinant Streptavidin (Recombinant Streptavidin; rStreptavidin)；重组链霉亲和素 |
| Synonyms | N/A |
| Purity | >98% by SDS-PAGE and HPLC analyses |
| Biological Activity | >17U/mg (one unit binds 1μg D－biotin) |
| A282 | 3.1 (0.1% solution) |
| Concentration | See label |
| Physical Appearance | Sterile colorless liquid |
| Formulation | A 0.2μm filtered concentrated sterile solution in ultrapure water. |
| Endotoxin | Less than 0.1EU/μg of rStreptavidin as determined by LAL method. |
| Reconstitution | － |
| Category | Antibody Purification |
| Background | Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.The molecular mass for the native protein as ~52kDa per tetramer, but under extreme denaturing conditions, giving a major band at about ~13kDa. Streptavidin has four binding sites for biotin, as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, or fluorochromes. However, avidin is highly basic (pI 10.5), compared to streptavidin (pI 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P5084-1mg | Recombinant Streptavidin | 1mg |
| P5084-5mg | Recombinant Streptavidin | 5mg |
| P5084-20mg | Recombinant Streptavidin | 20mg |
| P5084-100mg | Recombinant Streptavidin | 100mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少两年有效。由于蛋白的每次冻融均会引起部分失活，首次使用时建议分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
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文献和实验The overexpression of toxic recombinant proteins is often problematic, leading to either low production levels or inclusion bodies. Streptavidin is no exception and thus the highest production level reported to date for streptavidin is 70 mg
Colloidal Gold/Streptavidin Methods
. This nonspecific binding has been attributed both to its high isoelectric point (PI = 10) and to the fact that the protein is glycosylated (3 , 4 ). Because of the problem of nonspecific binding of avidin, streptavidin has largely replaced avidin
Smart Polymer-Streptavidin Conjugates
The conjugation of stimuli-responsive, or “smart,” polymers to streptavidin is described. The polymer is synthesized with a thiol-reactive end-group, which is used to end-graft the polymer to cysteine or lysine side-chains
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