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文献和实验Construction of Recombinant Human Cytomegalovirus
The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral
,并提高其生物活性,与普通的IGF-1或者LR3 IGF-1相比,OsrIGF-1的稳定性更高。同时,在GMP条件下生产的OsrIGF-1能保障批次间完全无差异,为实验和生产带来更高的保障。同时,OsrIGF-1批次生产可达克级别,源于高表达量的水稻胚乳平台,以满足大规模生产所需。通过实验验证,OsrIGF-1效能为人胰岛素的2000倍左右,活性与longR3相当,其在培养基中5μg/L的添加量对细胞增殖的效果好于人胰岛素10mg/L的添加量,大大减少了胰岛素的用量,降低生产成本。并且OsrIGF
Delivery of Recombinant Adenoviruses to Human Saphenous Vein
The human saphenous vein is the most commonly used conduit for coronary artery bypass grafting owing to its ready availability, ease of harvesting, and favorable surgical handling (1 ). However, it suffers from a progressive decline
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