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- 文献和实验
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- 保存条件:
低温保存
- 英文名:
M5 HiPer pTOPO-Blunt Cloning Kit 平末端克隆载体
- 库存:
167
- 供应商:
上海机纯实业有限公司
- 规格:
4x20T/20T
| 规格: | 4x20T | 产品价格: | ¥2747.0 |
|---|---|---|---|
| 规格: | 20T | 产品价格: | ¥747.0 |
告诉你M5 HiPer pTOPO-Blunt Cloning Kit 平末端克隆载体实验时您应该注意:
1、试剂不能与手接触。
2、试剂取用后一定要将瓶塞盖紧,不可放错瓶盖和滴管,绝不允许张冠李戴,用完后将瓶放回原处。
3、已取出的试剂不能再放回原试剂瓶内。
4、要用洁净的药勺,量筒或滴管取用试剂,绝对不准用同一种工具同时连续取用多种试剂。
5、取完一种试剂后,应将工具洗净、擦干后,方可取用另一种试剂。
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文献和实验Cloning via Restriction Digests
Steps for Cloning Prepare DNA. See Recovering Plasmids from your Stab Culture protocol if you need to prepare DNA from a bacterial stock. See PCR protocol if you are using PCR to generate an insert. Select
Cloning PCR products using TA vectors
] ligation kit from Pharmacia. Some netters have reported that some of these vectors have been giving them problems, and a few ways to avoid common pitfalls were suggested. For example, the pCR-Script [TM] vector used for blunt-end cloning
into dialysis tubing, and use of an elution trap. (See Moleclular Cloning for more details). Precipitate the DNA with NaOAc and EtOH, wash with 70% EtOH, and resuspend in TE or H2 O. Quantify and record the estimated DNA concentration of the isolated
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