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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
pETBLUE-2 BLUNT CLONING KIT
- 规格：
40RXN
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文献和实验3’-A overhang to each end of the PCR product. Blunt cloning vectors and directional TOPO® cloning technologies are designed to clone PCR products produced by proofreading polymerases. Successful cloning depends upon using the correct polymerase with your cloning
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
Blunt end cloning of PCR produc
% ethanol, dry and resuspend in 20 ul T4 PNK, 20 pmol ATP and 5-10 units T4 PNK. Incubate at 37 C for 1 hour. Ligation reaction: Blunt end ligation is carried out in the presence of 5% PEG 8000. A microgram of Bluescript SK+ is blunt ended (cut
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