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- 2026年06月02日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 保质期：
三年
- 供应商：
上海科渊生物技术有限公司
1.无需加热 反应在室温下进行
2.无异味 不含巯基乙醇
3.快速 膜再生时间 5-15 分钟
4.即用 无需配制
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文献和实验1) After ECL development, wash membrane once for 10min with PBST. 2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag for 30min at 50oC with occasional mixing. For low-abundance antigens, the strip
Probing and Stripping of Western Blot
270 ml H2OStripping the antibody from Western Blot(Natalya Yudkovsky and Jeff Ranish)Note: these two methods both work, but the first is less time consuming:Easy Stripping Method:1. Put the blot in the following solution
Stripping for reprobing western blots
minutes. (2) Discard buffer. (3) 5-10 minutes fresh stripping buffer. (4) Discard buffer. (5) 10 minutes PBS (6) 10 minutes PBS (7) 5 minutes TBST (8) 5 minutes TBST Ready
