Invitrogen CD3 Monoclonal Anti

Invitrogen CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), FITC, eBioscience™ 11-0030-81,11-0030-82,11-0030-85

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FIGURE 1/2

CD3 Antibody (11-0030-81)

PloS one 2012 - Figure 3 Inflammatory cell infiltrations and proliferation of the epithelium in the ureters of F2 mice showing hydronephrosis. Diseased ureters of BDF2 mice showed severe mononuclear cell infiltrations predominated by B220-positive cells (panel b). B220-positive cells and CD3-positive cells were mainly observed in the perivascular portions at the adventitia of ureters (panels a and b). CD16-positive cells w... View more
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数据图库

• 已发表图片 (1)
• 抗体检测数据 (1)

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FIGURE 2/2

CD3 Antibody (11-0030-81) in Flow

Staining of rat splenocytes with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.25 µg of Mouse IgG3 Isotype Control FITC (Product # 11-4742-42) (left) or 0.25 µg of Anti-Rat CD3 FITC (right). Cells in the lymphocyte gate were used for analysis.

产品细节

已验证应用
稀释倍数
流式细胞分析 (Flow)
0.5 µg/test
已发表应用
流式细胞分析 (Flow)
查看下方已发表的3篇文献
免疫组化 (IHC)
查看下方已发表的1篇文献
产品规格
种属反应
Rat
已发表种属
Rat
宿主/亚型
Mouse / IgG3, kappa
推荐的同型对照
Mouse IgG3 Isotype Control (B10), FITC, eBioscience™
分类
Monoclonal
类型
Antibody
克隆号
eBioG4.18 (G4.18)
偶联物
FITC
查看其它类型 
激发/发射光谱
查看光谱 
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.1% gelatin
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
RRID
AB_464877

产品详细信息

Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

引用文献 (4)

 

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排序依据 : 种属稀释倍数文章标题作者 杂志最近发表

• Flow (3)
• IHC (1)

BMC immunology

Renal allograft rejection, lymphocyte infiltration, and de novo donor-specific antibodies in a novel model of non-adherence to immunosuppressive therapy.

"11-0030 was used in Flow cytometry/Cell sorting to investigate the immunological mechanisms underlying chronic renal allograft rejection in a model of non-adherence to immunosuppressive therapy."

Authors Kühne L,Jung B,Poth H,Schuster A,Wurm S,Ruemmele P,Banas B,Bergler T

Species
Rat
Dilution
Not Cited
Year2017

Molecular medicine reports

IL-17 contributed to the neuropathic pain following peripheral nerve injury by promoting astrocyte proliferation and secretion of proinflammatory cytokines.

"11003082 was used in flow cytometry to examine T cell populations in a rat model of spinal nerve ligation"

Authors Sun C,Zhang J,Chen L,Liu T,Xu G,Li C,Yuan W,Xu H,Su Z

Species
Rat
Dilution
1:100
Year2017

Molecular vision

NK cell depletion delays corneal allograft rejection in baby rats.

"11-0030 was used in Flow cytometry/Cell sorting to compare immune cells responses to allogeneic keratoplasty after depletion of natural killer cells."

Authors Schwartzkopff J,Schlereth SL,Berger M,Bredow L,Birnbaum F,Böhringer D,Reinhard T

Species
Rat
Dilution
Not Cited
Year2010

生物信息学

蛋白别名: CD3 antigen delta polypeptide; CD3 antigen, gamma polypeptide; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, gamma polypeptide; CD3d; CD3g; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 gamma chain

基因别名: Cd3d; Cd3g; T3d

UniProt ID: (Rat) P19377, (Rat) Q64159

Entrez Gene ID: (Rat) 25710, (Rat) 300678

分子生物学功能:  cytokine receptor  defense/immunity protein  immunoglobulin receptor superfamily  receptor