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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Upon receipt, aliquot and store the Reduced BSA and BPDE-BSA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
- 库存:
100
- 规格:
96 assays
2 parts: 1 RT / 1 Blue Ice
存储温度:
Upon receipt, aliquot and store the Reduced BSA and BPDE-BSA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
检测方法:
Colorimetric
类别:
Kit
产品分类:
Oxidative Stress/ Damage->Protein Oxidation / Nitration->BPDE Protein Adduct ELISA
产品描述:
BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
联系方式:
生物商城:http://www.annoron.com
办公电话:010-56256916
官方售后:400-965-8633
企业 QQ:3212874516、3198592576
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文献和实验An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between n-6 fatty acid (FA)-derived lipid peroxide and lysine. Immunochemical and chemical methods can be used to detect the formation of HEL. For example, an ELISA
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
CellTrace CFSE Cell Proliferation Kit
实验材料 Kit Contents CellTrace™ CFSE (Component A), 10 vials, each containing 50 μg of lyophilized powder DMSO (Component B), 1 vial containing 0.5 mL of high quality dimethylsulfoxide
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