- ￥5832.53
- nsjbio
- V3571SAF-100UG
- 2025年07月13日
- Rabbit
- Human. Other species not known.
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human recombinant protein was used as the immunogen for the recombinant Integrin beta 3 antibody.
- 亚型：
Rabbit IgG, kappa
- 克隆性：
单克隆
- 适应物种：
Human. Other species not known.
- 宿主：
Rabbit
- 规格：
100 ug
Primary antibody
纯化方法：
Purified
通用信息：
1 mg/ml in 1X PBS; BSA free, sodium azide free
产品描述：
Reacts with human Integrin beta 3, also called CD61. It associates with the αV-chain (CD51) to form vitronectin receptor, or with the αIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.
联系方式：
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文献和实验for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
cells at 3000 X g. Resuspend in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition of beta
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