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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A portion of amino acids 49-83 from the mouse protein was used as the immunogen for the Cox4i1 antibody.
- 亚型:
Rabbit Ig
- 适应物种:
Mouse
- 宿主:
Rabbit
- 规格:
0.05 ml
Primary antibody
纯化方法:
Antigen affinity purified
通用信息:
In 1X PBS, pH 7.4, with 0.09% sodium azide
产品描述:
联系方式:
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Reagents Ammonium sulfate (50% [w/v]) (optional, see Step 23) Antibodies, primary Affinity-purified primary antibodies are diluted in PBS containing 1% BSA. The dilutions must be adjusted for each individual primary antibody. Typical
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
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