- ￥4980.18
- nsjbio
- R36465-100UG
- 2025年07月13日
- Goat
- Human, Mouse, Rat, Pig, Dog, Cow
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Amino acids NYPWYHQTSGSASH were used as the immunogen for this DLX2 antibody.
- 亚型：
Goat Ig
- 适应物种：
Human, Mouse, Rat, Pig, Dog, Cow
- 宿主：
Goat
- 规格：
100 ug
Primary antibody
纯化方法：
Antigen affinity purified
通用信息：
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
产品描述：
联系方式：
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
affinity) and the other is an IP kinase reaction. Substrate/Antibody, Lysate Interaction: 1. For GST c-Jun substrate interaction the quantity of protein on the beads is variable with the batch made (make fresh at least once a week). Generally
Immunoprecipitation and Immune Complex kinase assay
9) Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning and resuspending. 10) If doing kinase assay, do additional wash in TN and then proceed with kinase assay (below). 11) Pellets can be stored dry
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