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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A portion of amino acids 1460-1490 from the mouse protein was used as the immunogen for the Pi4ka antibody.
- 亚型:
Rabbit Ig
- 适应物种:
Mouse
- 宿主:
Rabbit
- 规格:
0.05 ml
Primary antibody
纯化方法:
Antigen affinity purified
通用信息:
In 1X PBS, pH 7.4, with 0.09% sodium azide
产品描述:
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
affinity) and the other is an IP kinase reaction. Substrate/Antibody, Lysate Interaction: 1. For GST c-Jun substrate interaction the quantity of protein on the beads is variable with the batch made (make fresh at least once a week). Generally
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