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Please store the product under the recommended conditions in the Certificate of Analysis.
- 英文名：
5-TMRIA
- 库存：
货期：14天
- 供应商：
MedChemExpress LLC
- CAS号：
114458-99-0
- 规格：
电询
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Tetramethylrhodamine-5-iodoacetamide
CAS No. : 114458-99-0
MCE 国际站：Tetramethylrhodamine-5-iodoacetamide
产品活性：Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 是一种选择性巯基反应染料，用于通过半胱氨酸残基对蛋白质进行非特异性标记。Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 可用于共价标记 DNA 片段。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios.
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文献和实验Labeling Gizzard Myosin with Tetramethylrhodamine Iodoacetamide (Primarily 17kDa Light Chain)
. 5.Resuspend pellets in 3-5 ml of buffer 2,centrifuge as in step 4. 6.Resuspend pellets in a small volume of buffer 3. 7.Dialyze overnight against buffer 4. 8.Centrifuge in a 50Ti rotor at 35,000 rpm,4℃,for 60 min.The supernatant is good for microinjection
LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
Day 1-2 Materials 1.0.5 M KCl,10 mM HEPES,pH 7.5,4℃,250 ml. 2.0.5 M KCl,50 mM HEPES,pH 8.0,4℃,250 ml. 3.Bio-Beads SM-2 in a 0.7x15 column. 4.20 mM KCl,20 mM PIPES,pH 7.0,4℃,250 ml. 5.IATR (tetramethylrhodamine iodoacetamide; Molecular Probes
LABELING MUSCLE ACTIN WITH IATR
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0 and 1000 ml for day 1. 2.100 mM KCl,100 mM boric acid,pH 8.4 at 4℃,10 ml. 3.IATR (tetramethylrhodamine iodoacetamide; Molecular Probes). 4.50Ti tubes
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