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VECTASHIELD Mounting Medium wi

th DAPI
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  • ￥3267
  • vector
  • H-1200
  • 2025年12月19日
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    上海研卉生物科技有限公司
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    • 供应商：

      上海研卉生物科技有限公司

    • 库存：

      大量

    • 英文名：

      VECTASHIELD Mounting Medium with DAPI

    • 规格：

      10 ml

    VECTASHIELD® Mounting Media are unsurpassed in preventing photobleaching. The different formulations of VECTASHIELD® Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. They are all compatible with fluorescein, Texas Red®, AMCA, DyLight® dyes, Alexa Fluor® dyes, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, histochemical stains, and most fluorochromes.

    Features of VECTASHIELD® Mounting Media:
    • Inhibit photobleaching of most fluorochromes
    • Available in non-hardening or hardening formulations
    • Available with or without DAPI or propidium iodide
    • No warming necessary
    • Slides can be viewed after prolonged storage
    • Continues to inhibit photobleaching after prolonged storage of mounted slides
    • Easy to use
    • Optically clear
    • Ideal refractive index

      The original VECTASHIELD® Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish. Mounted slides should be stored at 4 ºC.

      Both the VECTASHIELD® HardSet™ and the original VECTASHIELD® Mounting Media are available with or without the counterstain DAPI (4’, 6-diamidino-2-phenylindole). DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

      VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (located in product information box) to determine if VECTASHIELD® will be compatible in your system.

      Please note: VECTASHIELD® Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes.

      The refractive index for VECTASHIELD® Mounting Medium is 1.44.

      1R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.
     
     
     
     

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    图标文献和实验
    相关实验

    • 用DAPI进行细胞染色

      /0.5 ml in H2O and stocked at �20 in the darkStaining with antifade mounting solution:• Wash the cells twice with PBS• Mount with antifade solution with DAPI (Vectashield, Co#: H-1200，Vector Lab, 1.5 µg/ml) at 1:3 dilution with Vectashield H-1000

    • DAPI Counterstaining Protocols

      5 minutes.                 iv.  Rinse the sample several times in PBS. Drain excess buffer from the coverslip and mount.  We recommend using a mounting medium with an antifade reagent such as our SlowFade® Gold antifade reagent or ProLong® Gold

    • DAPI Nucleic Acid Stain

      , making certain that the cells are completely covered. C.     Incubate for 1–5 minutes. D.    Rinse the sample several times in PBS. Drain excess buffer from the coverslip and mount. We recommend using a mounting medium with an antifade

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