Hepatitis B virus(HBV)is a human pathogen,causing serious liver disease.The HBV surface protein antigens(HBsAg)are comprised of three carboxyl co terminal HBs proteins termed large(LHBs),middle(MHBs)and small(SHBs,also called major)protein.LHBs and MHBs also share the highly hydrophobic,repetitive,membrane spanning S domain.In addition,LHBs has a 119 amino acid region called preS1.
应用
1.Immunochromatography(capture and conjugate);2.Preparing monoclonal or polyclonal antibodies for HBsAg-preS1;3.ELISA.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
外观
Sterile Filtered White lyophilized(freeze-dried)powder.
纯度
>97%by SDS-PAGE and HPLC analyses.
来源
Escherichia coli.
成分
Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,pH 7.4,50 mM NaCl.
内毒素
Less than 1 EU/μg of rHBsAg-preS1 as determined by LAL method.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at<-20?C.Further dilutions should be made in appropriate buffered solutions.