重组人UBE2R1(带His标签)北京价格

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产品名称：重组人UBE2R1(带His标签)
英文名称：Recombinant Human Ubiquitin Conjugating Enzyme E2 R1, His
产品货号：GS4895
产品规格：10μg

分子量：Approximately 28.2kDa,a single non-glycosylated polypeptide chain containing 236 amino acids(a.a.)of human UBE2R1/CDC34 and 12 a.a.vector sequence including 6×His tag at N-terminus.
储存条件：冷冻（-20℃）

概述

Ubiquitin-conjugating enzyme E2 R1 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2R1 gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.UBE2R1 is important in the control of cell cycle and DNA replication.In vitro it catalyzes Lys-48-linked polyubiquitination.Additionally,the protein cooperates with the UBCH5C and the SCF ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Data is not available.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,1mMDTT,pH 7.5.
内毒素	Less than 1 EU/μg of rHuUBE2R1,His as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MHHHHHHAMG ILMARPLVPS SQKALLLELK GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP PNTYYEGGYF KARLKFPIDY PYSPPAFRFL TKMWHPNIYE TGDVCISILH PPVDDPQSGE LPSERWNPTQ NVRTILLSVI SLLNEPNTFS PANVDASVMY RKWKESKGKD REYTDIIRKQ VLGTKVDAER DGVKVPTTLA EYCVKTKAPA PDEGSDLFYD DYYEDGEVEE EA

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