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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Chondrolife Complete Med., 100
- 细胞类型:
人干细胞
- 物种来源:
人
- 器官来源:
人源
- 运输方式:
冷藏或冷冻
- 年限:
1-2年
- 生长状态:
贴壁生长
- 规格:
见说明书
Product Description
ChondroLife™ Complete Chondrogenesis Frozen Medium is a new medium optimized for the differentiation of Human Mesenchymal Stem Cells into chondrocytes.
In comparisons with commercially-available media, ChondroLife™ differentiation kit shows superior efficiency of differentiation as demonstrated by the grreater deposit of sulfated proteoglycans.
ChondroLife™ chondrogenic medium contains no antimicrobials and no phenol red. Antimicrobials are not required, but are recommended during differentiation due to the duration of culture and the frequency of handling of the cultures. Antimicrobials and phenol red may be purchased separately.
- Fully Supplemented: Offered as a convenient and easy to use, fully-supplemented complete frozen medium (100mL) to extend shelf-life.
Shown left: HMSC-Ad, passage 4, 4 days after inoculation with approximately 4,500 cells/cm2 (100X).
Shown below, left: Human Mesenchymal Stem Cells-Adult differentiated into Chondrocytes using ChondroLife™ and stained with Lifeline® Alcian Blue Staining Kit to visualize the proteoglycan deposition, post 3 weeks differentiation (100X).
Show below, right: HMSC-Ad differentiated into Chondrocytes using a competitor chondrogenesis differentiation kit and stained with Lifeline® Alcian Blue Staining Kit to visualize the proteoglycan deposition, post 3 weeks differentiation (100X).
ChondroLife™ Complete Chondrogenesis Medium is quality tested for:
- Sterility: Negative for bacterial and fungal growth
- pH: 7.6 +/- 0.3
- Cell Performance: Rate of proliferation and morphology
- Osmolality: 285 +/- 15 mOsm
- Endotoxin: < 0.5 EU/mL
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, and then centrifuged to pellet the bac teria. The pellet was resuspended or washed once in 100 µl of TE, centrifuged again, and resuspended in 10 µl of TE. The entire sample was added as the DNA template to a total 50 µl reaction volume. Choice of DNA Polymerases
to the animal and provides biologically meaningful samples. Only personnel with appropriate AHR training may handle live animals. Isoflourane inhalation: This should be given in sufficient dose to cause complete loss of breathing and pulse in the mouse. Apply










