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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存
- 保质期:
2年
- 英文名:
Long PfuMax DNA Polymerase
- 库存:
充足
- 供应商:
广州英赞生物科技有限公司
长片段PCR的福音——Long PfuMax DNA Polymerase
产品概述
产品内容
| 组分 | L136A | L136B |
| Long PfuMax DNA Polymerase | 200 U | 200 U× 5 |
| 2× Long PfuMax Buffer | 1.25 mL× 2 | 1.25 mL× 10 |
| 10mM dNTP mixture | 150 μL | 150 μL× 5 |
| 6× DNA Loading Buffer | 1 mL | 1 mL× 5 |
产品优势
高保真:保真度是Taq DNA聚合酶的80倍
灵敏度高:对低纯度模板有很强的耐受性,可高效扩增长片段DNA模板
实验案例
1. 超高的保真性
2. 优异的长片段扩增能力
应用范围
长片段PCR;高保真PCR;高GC PCR;困难PCR
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文献和实验vol 687 Chapter 2 采用DNA聚合酶融合技术进行长片段PCR扩增 (精华)
Methods in Molecular Biology vol.687 Park D.J. (ed.) PCR Protocols Chapter 2 PART II :Cloning and Sequencing Long-Range PCR with a DNA Polymerase Fusion Holly H. Hogrefe and Michael C. Borns Abstract Proofreading DNA
We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template
The Polymerase Chain Reaction (PCR)
. Did He Really Invent PCR? • The basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khorana in 1971:– Kleppe et al. (1971) J. Mol. Biol. 56, 341-346. • Progress was limited by primer synthesis and polymerase










