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- Lifeline Cell Technology
- 美国
- FC-0052
- 2026年01月20日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Normal Human Vas Deferens Fibroblasts
- 细胞类型：
人正常原代细胞
- 物种来源：
人源
- 器官来源：
人源
- 运输方式：
干冰或液氮
- 年限：
液氮储存10年以上
- 生长状态：
冻存
- 规格：
500,000 cells/vial
相关产品： 培养试剂 成纤维细胞培养基
Lifeline® Vas Deferens Fibroblasts, when grown in FibroLife® S2 culture medium, provide an excellent in vitro model for the study of male reproductive biology. Vas Defens Fibroblasts can be used as normal controls for cancer cell studies and are also ideal for the study of:
- androgen receptors
- reproductive cell morphogenesis
- fibrosis of the vas deferens
- other male urogenital disease states including male infertility
- toxicity and drug discovery
Vas Deferens Fibroblasts are plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest viability and plating efficiency. Vas Deferens Fibroblast Cells are cultured in FibroLife® S2 Medium.
Lifeline® cells need not be exposed to antimicrobials or phenol red when cultured in Lifeline® culture media. That’s an advantage as these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
500,000 cells per vial
Human Vas Deferens Fibroblasts are tested for:
- Proliferation: Population growth at various densities
- Cell Viability: 70% or greater when thawed from cryopreservation
- Morphology: Normal cell appearance for 15 population doublings
- Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth.
- Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
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文献和实验利用 Millicell 培养小室培养皮肤及肺类器官的实验方案
细胞的位置； 3) 皮下层，或皮下组织，主要包含脂肪细胞并起到保护下层组织的作用，可以作为能量储备，并通过绝缘提供一定的温度调节。 历史上，动物模型被用于测试化妆品的毒性和功效1 以及测试透皮药物吸收功效2。然而，出于伦理考虑，这些动物模型已逐渐被“零残忍”体外皮肤器官模型所取代，该模型使用人原代细胞及小室细胞培养来再现层状表皮结构。32013 年，欧盟率先全面禁止对化妆品进行动物试验。在此，我们将介绍一种利用人原代角质形成细胞 (PHK)、真皮成纤维细胞（人原代成纤维细胞，PHF），胶原蛋白涂层
Characterization of CGRP Receptor Binding
. Poyner, D.R., Taylor, G.M., Tomlinson, A.E., Richardson, A.G., and Smith, D.M. 1999 Characterization of receptors for calcitonin gene‐related peptide and adrenomedullin on the guinea‐pig vas deferens. Br. J
. Duct system The vas deferens connects the epididymis, in which sperm mature and ripen for up to 6 weeks, and the ejaculatory ducts. The seminal vesicles ― two convoluted membranous pouches ― secrete a viscous liquid
