- ￥11220
- Lifeline Cell Technology
- 美国
- FC-0049
- 2026年01月20日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Normal Human Lung Fibroblasts
- 细胞类型：
人正常原代细胞
- 物种来源：
人源
- 器官来源：
人源
- 运输方式：
干冰或液氮
- 年限：
液氮储存10年以上
- 生长状态：
冻存
- 规格：
500,000 cells/vial
Product Description
Lifeline® normal Human Primary Lung Fibroblasts provide an ideal cell system to:
- Study wound healing, toxicology, cancer, or basic cell biology.
- Use for drug screening, drug development, and genome editing applications.
- Establish serum free human feeder layers for human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and other cell culture applications requiring feeder layers.
Lifeline® Human Lung Fibroblasts have been isolated, plated and expanded in culture vessels as Primary Cells before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.
Our Lung Fibroblasts are quality tested in FibroLife® S2 or FibroLife® Xeno-Free Medium and demonstrate optimal growth at rates equal to or greater than classical serum-supplemented media. Fibroblasts are tested for:
- Proliferation and morphology: normal cell appearance for a period of at least 15 population doublings.
- Cell Viability: Minimum 70% viability when thawed from cryopreservation
- Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth.
- Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
Lifeline® human lung fibroblasts need not be exposed to antimicrobials or phenol red when cultured in FibroLife®Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
500,000 cells per vial
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文献和实验Isolation and Phenotypic Characterization of Lung Fibroblasts
such as the lung, heart, cornea, skin, and orbit. The tissue is mechanically dissociated and cells are allowed to proliferate from the fragments. Following establishment of a primary culture of fibroblasts, it is necessary to characterize the new strain of cells
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There is substantial evidence that aging is related to the finite ability of somatic cells to divide and repair damaged tissue. Since the seminal observation of Hayflick and Moor head (1 ) that cultured human fibroblasts have a finite
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Asthma is considered an airway inflammatory disorder characterized by variable airflow obstruction and airway hyperresponsiveness (1 ). The inflammatory component of asthma has been studied extensively over the past few years
