- ￥4475
- Leading Biology
- 1.56-100nmol/L
- EK00763E
- 美国
- 2025年11月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
安诺伦（北京）生物科技有限公司
- 检测范围：
1.56-100nmol/L
- 检测方法：
Spectrophotometrical
- 应用：
Competitive
- 适应物种：
General
- 样本：
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
- 规格：
96T
试剂盒组分：Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
功能：S-Adenosyl methionine (ademetionine, AdoMet, SAM, SAMe, SAM-e) is a common cosubstrate involved in methyl group transfers. SAM was first discovered in Italy by G. L. Cantoni in 1952. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase (EC 2.5.1.6). Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver.
储存条件：Store at +4°C. Please refer to protocols.
产品概述：General SAM/S-Adenosyl methionine ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of SAM in biological samples. Our rigorously validated, ready-to-go General SAM/S-Adenosyl methionine ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect SAM in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
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文献和实验Analysis of Oxidative Modification of Proteins
) 10 µM [3 H]methyl‐S‐adenosyl‐L‐methionine (5 to 15 Ci/mmol; [3 H] SAM; NEN) Protein‐L‐isoaspartyl methyltransferase (PIMT; Promega Corporation or purified from a known
, C) DNA-binding ELISA of the selected -6ZF (B) and -3ZF protein domains (C) expressed as fusions with MBP. All TFZF s were selected for VE-cadherin upregulation except 54.3, which was selected for ICAM-1 activation. The DNA substrates contained the 18
Guide to Cell Proliferation and Apoptosis Methods
1.3.2.1 Assays the measure plasma membrane leakage 51 Cytotoxicity Detection Kit (LDH) 52 Cellular DNA Fragmentation ELISA 54
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