- ￥2475
- Leading Biology
- 0.78-50 ng/mL
- EK00039E
- 美国
- 2025年11月12日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
安诺伦（北京）生物科技有限公司
- 检测范围：
0.78-50 ng/mL
- 检测方法：
Spectrophotometrical
- 应用：
Competitive
- 适应物种：
General
- 样本：
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
- 规格：
96T
试剂盒组分：Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
功能：Cortisol (hydrocortisone) is a steroid hormone, more specifically a glucocorticoid, produced by the zona fasciculata of the adrenal gland. It is released in response to stress and a low level of blood glucocorticoids. Its primary functions are to increase blood sugar through gluconeogenesis; suppress the immune system; and aid in fat, protein and carbohydrate metabolism. It also decreases bone formation.
储存条件：Store at +4°C. Please refer to protocols.
产品概述：General Cortisol/Cortisol ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of Cortisol in biological samples. Our rigorously validated, ready-to-go General Cortisol/Cortisol ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect Cortisol in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
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文献和实验Measuring Endocrine (Cortisol) Responses of Zebrafish to Stress
hormone, both humans and zebrafish utilize cortisol. This protocol explains the whole-body cortisol extraction procedure and the use of the human salivary cortisol ELISA kit to measure the amount of cortisol in each zebrafish sample
Methods to Quantify Basal and Stress-Induced Cortisol Response in Larval Zebrafish
the previously characterized cortisol RIA for adult zebrafish while offering an alternative to the ELISA method. We also provide a methodology for the use of an acute stress paradigm customized for zebrafish larvae, i.e., acute mild
人皮质醇（ Cortisol ） 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 皮质醇（ Cortisol ） 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 人皮质醇（ Cortisol ） 水平。用纯化的 人皮质醇（ Cortisol ） 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入
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