- ￥495
- Leading Biology
- APR02941G
- 2025年10月26日
- WB, IHC-P, E
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 适应物种：
Human, Mouse
- 库存：
100
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, E
- 靶点：
SENP1 Antibody detects endogenous levels of total SENP1 protein.
- 规格：
50 μl
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文献和实验the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
inhibitors, and aliquot it for use. Then freeze the aliquots and keep just one around for day to day use at four degrees C. Raw serum containing antibodies: Aliquot into reasonable aliquots (usually 10-50 ml) appropriate to your use of the antibody
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