- $495
- Leading Biology
- AMM00669G
- 2025年10月12日
- IF, FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse / IgG1, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 库存：
100
- 宿主：
Mouse
- 应用范围：
IF, FC
- 规格：
100 μg
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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
Blocking of unwanted non-specific staining
Source of unwanted staining, besides poor knowledge of the antibody reactivity and malice, is due to: Endogenous enzymes or fluorochromes. Endogenous biotin. Endogenous antibody binding activity (Fc
Antigen Design Sera Purification Tech Sheet
determination, protein homology studies and protein purification. While it is quite easy to generate anti-peptide antibodies, it is important to carefully consider the ultimate use for the antibody and the sequence used to ensure success. This tech sheet
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